Influenza A H3N2 Hemagglutinin / HA Antibody, Mouse MAb

RRID Number: AB_2860294

Influenza A H3N2 Hemagglutinin / HA Antibody, Mouse MAb (Mouse Monoclonal antibody) General Information

Product name
Influenza A H3N2 Hemagglutinin / HA Antibody, Mouse MAb
Validated applications
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
H3N2 Hemagglutinin/HA
Has cross-reactivity in ELISA with
H7N7 (A/Netherlands/219/03) HA
H3N2 (A/Wyoming/03/2003) HA
H3N2 (A/Aichi/2/1968) HA
No cross-reactivity in ELISA with
H1N1 (A/California/04/2009) HA
H1N1 (A/California/07/2009) HA
H1N1 (A/Brisbane/59/2007) HA
H5N1 (A/Viet nam/1194/2004) HA
H5N1 (A/Indonesia/5/2005) HA
H5N1 (A/turkey/Turkey/1/2005) HA
H5N1 (A/Anhui/1/2005)HA
H5N1 (A/bar-headed goose/Qinghai/14/2008) HA
H9N2 (A/Hong Kong/1073/99) HA
H2N2 (A/Japan/305/1957) HA
Influenza B (B/Florida/04/2006) HA
Human cell lysate (293 cell line)
Recombinant H3N2 HA protein (Catalog#11056-V08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H3N2 Hemagglutinin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #MM03
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Influenza A H3N2 Hemagglutinin / HA Antibody, Mouse MAb (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect H3N2 HA in WB. Using a DAB detection system, the detection limit for H3N2 HA is approximately 20 ng/lane under non-reducing conditions and 8 ng/lane under reducing conditions.
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Hemagglutinin/HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
  • White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
  • Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
  • Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018
  • Hemagglutinin stalk domain from H5N1 strain as a potentially universal antigen.
    K Uranowska, J Tyborowska, A Jurek…
    Acta biochimica polonica
  • Colorimetric detection of influenza A virus using antibody-functionalized gold nanoparticles
    Liu, Y;Zhang, L;Wei, W;Zhao, H;Zhou, Z;Zhang, Y;Liu, S;
    the mono-clonal anti-HA antibody (mAb) based specific recognition, to create mAb–AuNP probes for the colorimetric detection of H3N2 IAV (A/Brisbane/10/2007).
  • Generation of Immunity against Pathogens via Single-Domain Antibody-Antigen Constructs
    Duarte, JN;Cragnolini, JJ;Swee, LK;Bilate, AM;Bader, J;Ingram, JR;Rashidfarrokhi, A;Fang, T;Schiepers, A;Hanke, L;Ploegh, HL;
    J. Immunol.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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