Cat: 100028-MM10
Application | Dilution |
---|---|
WB | 1/2000-1/5000 |
ELISA | 1/2000-1/5000 |
ICC-IF | 1/200-1/500 |
IP | 2-8 μL/mg of lysate |
Anti-HA Tag was immunoprecipitated using:
Lane A: HA-ARG1-myc transfected 293 cell lysate (0.5mg)
Lane B: myc-ARG1-HA transfected 293 cell lysate (0.5mg)
2 μL anti-HA Tag mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.
Primary antibody:
Anti-HA Tag rabbit monoclonal antibody, at 1:100 dilution
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Anti-HA Tag mouse monoclonal antibody at 1:1000 dilution
Lane A: HA-GST (Recombinant protein)(30ng)
Lane B: GST-HA (Recombinant protein)(10ng)
Lane C: HA-ARG1-myc transfected 293 cell lysate (2ug)
Lane D: HA-mFABP4-myc transfected 293 cell lysate (2ug)
Lane E: myc-mFABP4-HA transfected 293 cell lysate (0.5ug)
Lane F: myc-ARG1-HA transfected 293 cell lysate (2ug)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
High-throughput Recombinant Antibody Production