Anti-GST tag Antibody, Mouse Monoclonal
Cat: 11213-MM01
Anti-GST tag Antibody, Mouse Monoclonal General Information
No cross-reactivity in ELISA with
E.coli cell lysate
Anti-GST tag Antibody, Mouse Monoclonal Validated Applications
| Application | Dilution |
|---|---|
| WB | 1/1000-1/10000 |
| IP | 1-4 μl/mg of lysate |
Anti-GST tag Antibody, Mouse Monoclonal Images
Anti-GST Tag was immunoprecipitated using:
Lane A: GST-ARG1-Flag transfected 293 cell lysate (0.5mg)
Lane B: Flag-ARG1-GST transfected 293 cell lysate (0.5mg)
2 μg anti-HA Tag mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.
Primary antibody:
Anti-GST Tag rabbit monoclonal antibody, at 1:100 dilution
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Recognize GST tag fusion protein human ERK2.
Anti-GST Tag mouse monoclonal antibody at 1:4000
Lane A: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (100ng)
Lane B: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (50ng)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Recognize GST tag fusion protein human USP7.
Anti-GST Tag mouse monoclonal antibody at 1:4000
Lane A: GST-USP7 (Recombinant protein, Cat#11681-H20B) (100ng)
Lane B: GST-USP7 (Recombinant protein, Cat#11681-H20B) (50ng)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Recognize GST tag fusion proteins which expressed by 293 cell.
Anti-GST Tag mouse monoclonal antibody at 1:1250 or 1:2500
Lane A: GST-FABP4-flag transfected 293 Cell Lysate (30ug)
Lane B: flag-FABP4-GST transfected 293 Cell Lysate (30ug)
Lane C: flag-AGR1-GST transfected 293 Cell Lysate (30ug)
Lane D: Negative control
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Recognize GST tag fusion proteins which expressed by E. coli.
Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250
Lane A: GST-mBID transfected E.coli cell lysate (0.5ug)
Lane B: GST-mBID transfected E.coli cell lysate (0.2ug)
Lane C: GST-mBID transfected E.coli cell lysate (0.1ug)
Lane D: GST-mBID transfected E.coli cell lysate (0.05ug)
Lane E: GST-mBID transfected E.coli cell lysate (0.02ug)
Lane F: GST-YWHAB transfected E.coli cell lysate (0.5ug)
Lane G: GST-YWHAB transfected E.coli cell lysate (0.2ug)
Lane H: GST-YWHAB transfected E.coli cell lysate (0.1ug)
Lane I: GST-YWHAB transfected E.coli cell lysate (0.05ug)
Lane J: GST-YWHAB transfected E.coli cell lysate (0.02ug)
Lane K: Empty vecter (0.5ug)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:1000
Lane A: GST-mBID transfected E.coli cell lysate (0.1ug)
Lane B: GST-YWHAB transfected E.coli cell lysate (0.1ug)
Lane C: Empty vecter (0.1ug)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:25000
Lane A: GST-mBID transfected E.coli cell lysate (0.2ug)
Lane B: GST-YWHAB transfected E.coli cell lysate (0.2ug)
Lane C: Empty vecter (0.2ug)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
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