Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal

Anti-GFP Tag was immunoprecipitated using:

Lane A: GFP transfected E.coli cell lysate (0.5mg)

Lane B: GFP transfected 293 cell lysate (0.5mg)

Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-GFP rabbit monoclonal antibody at 1:500 dilution

Lane A: GFP transfected E.coli lysate (5ug)

Lane B: GFP transfected 293 Cell lysate (30ug)

Lane C: Non-transfected 293 Cells lysate (30ug)

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:27 kDa

Observed band size:27 kDa