Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal
EliteRmab®, manufactured by recombinant technology
Cat: 13105-R208
Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal General Information
Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal Validated Applications
| Application | Dilution |
|---|---|
| WB | 1/1000-1/5000 |
| ELISA | 1/10000-1/20000 |
| IP | 1-4 μl/mg of lysate |
Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal Images
Anti-GFP Tag was immunoprecipitated using:
Lane A: GFP transfected E.coli cell lysate (0.5mg)
Lane B: GFP transfected 293 cell lysate (0.5mg)
Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G.
Primary antibody:
Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Anti-GFP rabbit monoclonal antibody at 1:500 dilution
Lane A: GFP transfected E.coli lysate (5ug)
Lane B: GFP transfected 293 Cell lysate (30ug)
Lane C: Non-transfected 293 Cells lysate (30ug)
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:27 kDa
Observed band size:27 kDa
GFP Tag Background Information
- Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
- Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.
- Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.
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