Anti-CD8 alpha Antibody

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Anti-CD8 alpha Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-CD8 alpha Antibody
Validated applications
ELISA,ELISA(Det)
Species reactivity
Reacts with: Ferret
Specificity
Ferret CD8 alpha
No cross-reactivity in ELISA with
Human CD8A
Mouse CD8A
Human cell lysate (293 cell line)
Immunogen
Recombinant Ferret CD8a protein (Catalog#60001-F08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret CD8a (rF CD8a; Catalog#60001-F08H; ABS50091.1; Met 1-Glu 186). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #3B5H2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD8 alpha Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:1000-1:2000
ELISA(Det) 1:1000-1:10000
Notes
ELISA(Det): This antibody will detect Ferret CD8 / CD8 alpha / Leu-2 in ELISA pair set (Catalog: # SEK60001). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 60001-R070).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

CD8 alpha Background Information

T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Full Name
CD8a molecule
References
    References Devine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.

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