Cat: 101292-T38
Application | Dilution |
---|---|
WB | 1:500-1:2000 |
ICC-IF | 1:300-1:10000 |
IP | 0.5-2 μg/mg of lysate |
E-tag was immunoprecipitated using:
Lane A:0.5 mg E tag-1M-125D-His© Whole Cell Lysate
Lane B:0.5 mg His-E tag-1M-125D© Whole Cell Lysate
Lane C:0.5 mg His-1M-125D-E tag© Whole Cell Lysate
Lane D:0.5 mg E tag-1M-244P-His© Whole Cell Lysate
Lane E:0.5 mg Psetp2 Whole Cell Lysate
2 µL anti-E-tag rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.
Primary antibody:
Anti-E-tag rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.
Performed under reducing conditions.
Anti-E Tag rabbit polyclonal antibody at 1:1000 dilution.
Lane A: Etag-GFERh(C) transfected 293 cell lysate at 2ug
Lane B: his-Etag-GFER(C) transfected 293 cell lysate at 2ug
Lane C: his-GFER-Etag(C) transfected 293 cell lysate at 2ug
Lane D: Etag-GSTT2h(C) transfected 293 cell lysate at 10ug
Lane E: his-GSTT2-Etag(C) transfected 293 cell lysate at 10ug
Lane F: his-Etag-GSTT2(C) transfected 293 cell lysate at 20ug
Lane G: Nagetive control at 10ug
Secondary
Dylight 800-labeled Antibody To Rabbit IgG (H+L) at 1/5000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
High-throughput Recombinant Antibody Production