Anti-E-tag Antibody

Immunofluorescence staining of E-Tag in Hela cells, transfected with pSTEP2-Etag-GFERh (Figure A), pSTEP2-his-Etag-GFER (Figure B), pSTEP2-his-GFER-Etag (Figure C), pSTEP2-Etag-GSTT2h (Figure D),pSTEP2-his-Etag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Etag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-E-Tag polyclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

E-tag was immunoprecipitated using:

Lane A:0.5 mg E tag-1M-125D-His© Whole Cell Lysate

Lane B:0.5 mg His-E tag-1M-125D© Whole Cell Lysate

Lane C:0.5 mg His-1M-125D-E tag© Whole Cell Lysate

Lane D:0.5 mg E tag-1M-244P-His© Whole Cell Lysate

Lane E:0.5 mg Psetp2 Whole Cell Lysate

2 µL anti-E-tag rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-E-tag rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Anti-E Tag rabbit polyclonal antibody at 1:1000 dilution.

Lane A: Etag-GFERh(C) transfected 293 cell lysate at 2ug

Lane B: his-Etag-GFER(C) transfected 293 cell lysate at 2ug

Lane C: his-GFER-Etag(C) transfected 293 cell lysate at 2ug

Lane D: Etag-GSTT2h(C) transfected 293 cell lysate at 10ug

Lane E: his-GSTT2-Etag(C) transfected 293 cell lysate at 10ug

Lane F: his-Etag-GSTT2(C) transfected 293 cell lysate at 20ug

Lane G: Nagetive control at 10ug

Secondary

Dylight 800-labeled Antibody To Rabbit IgG (H+L) at 1/5000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.