SARS-CoV-2 (2019-nCoV) Spike Non-Neutralizing Antibody, Rabbit MAb


SARS-CoV-2 (2019-nCoV) Spike Non-Neutralizing Antibody, Rabbit MAb General Information

Product name
SARS-CoV-2 (2019-nCoV) Spike Non-Neutralizing Antibody, Rabbit MAb
Validated applications
SARS-CoV-2 Coronavirus spike
Has cross-reactivity in ELISA with
SARS-CoV-2 (2019-nCoV) Spike S1-His Protein (Catalog#40591-V08B1, Catalog#40591-V08H)
SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Protein (Catalog#40589-V08B1)
SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein(Catalog#40592-V05H)
SARS-CoV Spike S1-His Protein (Catalog#40150-V08B1)
No cross-reactivity in ELISA with
SARS-CoV-2 (2019-nCoV) Spike S2 ECD-His Recombinant Protein (Cat# 40590-V08B)
MERS-CoV Spike S1 Protein (Cat# 40069-V08B1)
HCoV-HKU1 (isolate N5) Spike S1 Protein (Cat# 40602-V08H)
HCoV-HKU1 (isolate N1) Spike S1 Protein (Cat# 40021-V08H)
HCoV-229E Spike S1 Protein (Cat# 40601-V08H)
HCoV-NL63 Spike S1 Protein (Cat# 40600-V08H)
HCoV-OC43 Spike S1+S2 ECD Protein (Cat# 40607-V08B)
Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein (Catalog#40592-V05H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein (Catalog#40592-V05H; YP_009724390.1; Arg319-Phe541). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Rabbit IgG Clone #493
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV-2 (2019-nCoV) Spike Non-Neutralizing Antibody, Rabbit MAb Validated Applications

Application Dilution
WB 1:5000-1:20000
ELISA 1:5000-1:10000
Neutralization: It's Non-Neutralizing Antibody, which been measured by competitive ELISA and pseudovirus microneutralization assay in vitro.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV-2 (2019-nCoV) Spike Non-Neutralizing Antibody, Rabbit MAb Images

Anti-SARS-CoV-2 (2019-nCoV) Spike rabbit monoclonal antibody at 1:2000 dilution.

Lane A: SARS-COV-2 Spike overexpressed HEK293 Whole Cell Lysate

Lane B: HEK293 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Coronavirus spike Background Information

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.