Human coronavirus (HCoV-HKU1)Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified

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Human coronavirus (HCoV-HKU1)Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified General Information

Product name
Human coronavirus (HCoV-HKU1)Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified
Validated applications
WB,ELISA
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
HCoV-HKU1 Coronavirus Nucleocapsid
Immunogen
Recombinant Human coronavirus (HCoV-HKU1) Nucleoprotein / NP Protein (Catalog#40642-V07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human coronavirus (HCoV-HKU1) Nucleoprotein / NP ( Catalog#40642-V07E; YP_173242.1; Met1-Ala441). Coronavirus Nucleocapsid specific IgG was purified by Human coronavirus (HCoV-HKU1) Nucleoprotein / NP affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human coronavirus (HCoV-HKU1)Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified Validated Applications

Application Dilution
WB 1:2000-1:10000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Human coronavirus (HCoV-HKU1)Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified Images

Anti-HCoV HKU1 NP rabbit polyclonal antibody at 1:2000 dilution.

Sample:Human coronavirus (HCoV-HKU1) Nucleoprotein / NP Protein

Lane A: 5ng

Lane B: 1ng

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Coronavirus Nucleocapsid Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.

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