SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit Mab
Cat: 40588-R0002
SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit Mab General Information
Product name
SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit Mab
Validated applications
WB,ELISA,ELISA(Cap)
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated.
(Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
SARS-CoV-2 Coronavirus Nucleocapsid
Has cross-reactivity in ELISA and WB with
SARS-CoV Nucleocapsid Protein (His Tag) (Cat# 40143-V08B)
No cross-reactivity in ELISA with
MERS-CoV Nucleocapsid Protein (His Tag) (Cat# 40068-V08B)
HCoV-229E Nucleoprotein protein (40640-V07E)
HCoV-NL63 Nucleoprotein protein (40641-V07E)
HCoV-HKU1(isolate N5) Nucleoprotein protein (40642-V07E)
HCoV-OC43 Nucleoprotein (40643-V07E)
Has cross-reactivity in ELISA and WB with
SARS-CoV Nucleocapsid Protein (His Tag) (Cat# 40143-V08B)
No cross-reactivity in ELISA with
MERS-CoV Nucleocapsid Protein (His Tag) (Cat# 40068-V08B)
HCoV-229E Nucleoprotein protein (40640-V07E)
HCoV-NL63 Nucleoprotein protein (40641-V07E)
HCoV-HKU1(isolate N5) Nucleoprotein protein (40642-V07E)
HCoV-OC43 Nucleoprotein (40643-V07E)
Immunogen
Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Catalog#40588-V08B)
Preparation
This product is a recombinant monoclonal antibody expressed from HEK293 cells.
Source
Monoclonal Rabbit IgG Clone #0002
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit Mab Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:1000-1:5000 |
| ELISA | 1:5000-1:10000 |
| ELISA(Cap) | 1:250-1:2000 |
Notes
| ELISA(Cap)： | In a sandwich ELISA, Cat# 40588-R0002 can be used as capture antibody when paired with Cat# 40588-R0006-1 |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit Mab Images
Anti-SARS-NP rabbit monoclonal antibody at 1:1000 dilution.
Lane A: SARS-CoV NP Protein (Cat#40143-V08B) (10ng)
Lane B: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08H) (10ng)
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Coronavirus Nucleocapsid Background Information
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
- 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
- Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
- Li W. et al., 2005, Science. 309 (5742): 1864-8.
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