SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Mouse MAb

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SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Mouse MAb General Information

Product name
SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Mouse MAb
Validated applications
WB,ELISA,ELISA(Det),IHC-P,ICC/IF,IP
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
SARS-CoV-2 Coronavirus Nucleocapsid
Immunogen
Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Catalog#40588-V08B)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, SARS-CoV-2 (2019-nCoV) Nucleocapsid (Catalog#40588-V08B; YP_009724397.2(335Gly/Ala); Met1-Ala419). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #128
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Mouse MAb Validated Applications

Application Dilution
WB 1:2000-1:5000
ELISA 1:1000-1:2000
IHC-P 1:50000-1:200000
ICC-IF 1:20-1:100
IP IP:1-5μL/mg of lysate
ELISA(Det) 1:1000-1:10000
Notes
ELISA(Det): In a sandwich ELISA, Cat# 40588-MM128 can be used as detection antibody when paired with Cat# 40588-MM122 + 40588-MM124. In a sandwich ELISA, Cat# 40588-MM128 can be used as detection antibody when paired with Cat# 40588-MM137 + 40143-MM05.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Mouse MAb Images

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid mouse monoclonal antibody at 1:2000 dilution.

Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell Lysate

Lane B: HEK293 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

SARS-COV-2 Nucleocapsid was immunoprecipitated using:

Lane A:0.5 mg SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell Lysate

Lane B:0.5 mg HEK293 Whole Cell Lysate

4 µL anti- SARS-COV-2 Nucleocapsid rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti- SARS-COV-2 Nucleocapsid rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Immunochemical analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS-CoV-2 Nucleocapsid Mouse Mab,then a HRP-conjugated second step antibody.
Immunofluorescence analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS-CoV-2 Nucleocapsid Mouse Mab,then a Alexa Fluor®488-conjugated second step antibody.

Coronavirus Nucleocapsid Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.

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