SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified

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SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified General Information

Product name
SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified
Validated applications
WB,ELISA
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
SARS Coronavirus Nucleocapsid
Has cross-reactivity in ELISA and WB with
SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Protein (Cat# 40588-V08B).
Immunogen
Recombinant SARS-CoV Nucleoprotein / NP Protein (Catalog#40143-V08B)
Preparation
Produced in rabbits immunized with purified, recombinant SARS-CoV Nucleoprotein / NP (Catalog#40143-V08B; NP_828858.1; Met1-Ala422). SARS-CoV Nucleoprotein / NP specific IgG was purified by SARS-CoV Nucleoprotein / NP affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified Validated Applications

Application Dilution
WB 1:1000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified Images

Anti-SARS-NP rabbit polyclonal antibody at 1:2000 dilution.

Lane A: SARS-CoV NP Protein (Cat#40143-V08B) (30ng)

Lane B: SARS-CoV NP Protein (Cat#40143-V08B) (5ng)

Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (30ng)

Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (5ng)

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Citation List

SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified: Synonyms

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
  • Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model
    Author
    Rockx, B;Kuiken, T;Herfst, S;Bestebroer, T;Lamers, MM;Oude Munnink, BB;de Meulder, D;van Amerongen, G;van den Brand, J;Okba, NMA;Schipper, D;van Run, P;Leijten, L;Sikkema, R;Verschoor, E;Verstrepen, B;Bogers, W;Langermans, J;Drosten, C;Fentener van Vlissingen, M;Fouchier, R;de Swart, R;Koopmans, M;Haagmans, BL;
    Year
    2020
    Journal
    Science
    Application
    IHC
  • Evidence for gastrointestinal infection of SARS-CoV-2
    Author
    Xiao, F;Tang, M;Zheng, X;Liu, Y;Li, X;Shan, H;
    Year
    2020
    Journal
    Gastroenterology
  • Renal histopathological analysis of 26 postmortem findings of patients with COVID-19 in China
    Author
    Su, H;Yang, M;Wan, C;Yi, LX;Tang, F;Zhu, HY;Yi, F;Yang, HC;Fogo, AB;Nie, X;Zhang, C;
    Year
    2020
    Journal
    Kidney Int.
    Application
    IF
  • Generation of a Broadly Useful Model for COVID-19 Pathogenesis, Vaccination, and Treatment
    Author
    Sun, J;Zhuang, Z;Zheng, J;Li, K;Wong, R;Liu, D;Huang, J;He, J;Zhu, A;Zhao, J;Li, X;Xi, Y;Chen, R;Alshukairi, A;Chen, Z;Zhang, Z;Chen, C;Huang, X;Li, F;Lai, X;Chen, D;Wen, L;Zhuo, J;Zhang, Y;Wang, Y;Huang, S;Dai, J;Shi, Y;Zheng, K;Leidinger, M;Chen, J;Li, Y;Zhong, N;Meyerholz, D;McCray, P;Perlman, S;Zhao, J;
    Year
    2020
    Journal
    Cell
    Application
    FFA

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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