SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (HRP), Rabbit MAb

RRID Number: AB_2857929
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SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (HRP), Rabbit MAb General Information

Product name
SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (HRP), Rabbit MAb
Validated applications
ELISA
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
SARS Coronavirus Nucleocapsid
Immunogen
Recombinant SARS-CoV Nucleoprotein / NP Protein (Catalog#40143-V08B)
Preparation
The unconjugated product is a recombinant monoclonal antibody expressed from HEK293 cells. Then it been conjugated with horseradish-peroxidase (HRP).
Source
Monoclonal Rabbit IgG Clone #040
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
HRP
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Protected from prolonged exposure to light. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (HRP), Rabbit MAb Validated Applications

Application Dilution
ELISA 0.1-1 μg/ml
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Citation List

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (HRP), Rabbit MAb: Synonyms

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.

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