SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb

RRID Number: AB_2827973
COVID-19 Nucleocapsid Research.
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SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb General Information

Product name
SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb
Validated applications
WB,ELISA
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
2019-nCoV Coronavirus Nucleocapsid
Has cross-reactivity in ELISA and WB with
SARS-CoV Nucleocapsid Protein (Cat# 40143-V08B)
No cross-reactivity in ELISA with
MERS-CoV Nucleoprotein protein (40068-V08B)
HCoV-229E Nucleoprotein protein (40640-V07E)
HCoV-NL63 Nucleoprotein protein (40641-V07E)
HCoV-HKU1(isolate N5) Nucleoprotein protein (40642-V07E)
HCoV-OC43 Nucleoprotein (40643-V07E)
Preparation
This product is a recombinant monoclonal antibody expressed from HEK293 cells.
Source
Monoclonal Rabbit IgG Clone #019
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb Validated Applications

Application Dilution
WB 1:1000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb Images

Anti-2019-nCoV NP rabbit monoclonal antibody at 1:1000 dilution.

Sample: 2019-nCoV NP Protein (Cat# 40588-V08B)

Lane A: 30ng

Lane B: 10ng

Lane C: 5ng

Lane D: 2ng

Lane E: 1ng

Lane F: 0.5ng

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb: Synonyms

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-NCP-CoV Nucleocapsid Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-np Antibody; Anti-nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
  • Collapsing Glomerulopathy in a Patient With Coronavirus Disease 2019 (COVID-19)
    Author
    Larsen, CP;Bourne, TD;Wilson, JD;Saqqa, O;Sharshir, MA;
    Year
    2020
    Journal
    Kidney Int Rep
    Application
    IHC
  • Proteomics of SARS-CoV-2-infected host cells reveals therapy targets
    Author
    Bojkova, D;Klann, K;Koch, B;Widera, M;Krause, D;Ciesek, S;Cinatl, J;Münch, C;
    Year
    2020
    Journal
    Nature
    Application
    ViraM infection assay
  • The Novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Directly Decimates Human Spleens and Lymph Nodes
    Author
    Feng, Z;Diao, B;Wang, R;Wang, G;Wang, C;Tan, Y;
    Year
    2020
    Journal
    medRxiv
  • Development of an Inactivated Vaccine Candidate, BBIBP-CorV, with Potent Protection against SARS-CoV-2
    Author
    Wang, H;Zhang, Y;Huang, B;Deng, W;Quan, Y;Wang, W;Xu, W;Zhao, Y;Li, N;Zhang, J;Liang, H;Bao, L;Xu, Y;Ding, L;Zhou, W;Gao, H;Liu, J;Niu, P;Zhao, L;Zhen, W;Fu, H;Yu, S;Zhang, Z;Xu, G;Li, C;Lou, Z;Xu, M;Qin, C;Wu, G;Gao, G;Tan, W;Yang, X;
    Year
    2020
    Journal
    Cell
    Application
    WB
  • Sofosbuvir protects human brain organoids against SARS-CoV-2
    Author
    Mesci, P;Macia, A;Saleh, A;Martin-Sancho, L;Yin, X;Snethlage, C;Avansini, S;Chanda, S;Muotri, A;
    Year
    2020
    Journal
    bioRxiv
    Application
    IF
  • 上海 2 株严重急性呼吸综合征冠状病毒 2 的分离与鉴定
    Author
    彭浩然, ;李成忠, ;唐海琳, ;薛建亚, ;陈志辉, ;梁雪松, ;朱咏梅, ;夏炳辉, ;朱勇喆, ;吴俊杰, ;赵兰娟, ;任浩, ;江亮亮, ;何燕华, ;戚中田, ;赵平, ;
    Year
    2020
    Journal
    第二军医大学学报
  • SARS-CoV-2 growth, furin-cleavage-site adaptation and neutralization using serum from acutely infected, hospitalized COVID-19 patients
    Author
    Klimstra, W;Tilston-Lunel, N;Nambulli, S;Boslett, J;McMillen, C;Gilliland, T;Dunn, M;Sun, C;Wheeler, S;Wells, A;Hartman, A;McElroy, A;Reed, D;Rennick, L;Duprex, W;
    Year
    2020
    Journal
    bioRxiv
    Application
    Immunoplaque assay
  • Saxifraga spinulosa-Derived Components Rapidly Inactivate Multiple Viruses Including SARS-CoV-2
    Author
    Takeda, Y;Murata, T;Jamsransuren, D;Suganuma, K;Kazami, Y;Batkhuu, J;Badral, D;Ogawa, H;
    Year
    2020
    Journal
    Viruses

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