SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb

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SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb General Information

Product name
SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb
Validated applications
WB,ELISA,IHC-P,ICC/IF
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
SARS Coronavirus Nucleocapsid
Has cross-reactivity in ELISA with
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Cat# 40588-V08B)
No cross-reactivity in ELISA with
MERS-CoV Nucleoprotein protein (40068-V08B)
HCoV-229E Nucleoprotein protein (40640-V07E)
HCoV-NL63 Nucleoprotein protein (40641-V07E)
HCoV-HKU1(isolate N5) Nucleoprotein protein (40642-V07E)
HCoV-OC43 Nucleoprotein (40643-V07E)
Immunogen
Recombinant SARS-CoV Nucleoprotein / NP Protein (Catalog#40143-V08B)
Preparation
It is a chimeric monoclonal antibody combining the human IgG1 Fc molecule with mouse scFv, and the variable region was from mouse monoclonal antibody (Cat#40143-MM08). The chimeric monoclonal antibody was produced using recombinant antibody technology.
Source
Monoclonal Mouse scFv / human IgG1 Fc Clone #08
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb Validated Applications

Application Dilution
WB 1:2000-1:5000
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb Images

Anti-SARS-CoV / SARS-CoV-2 Nucleocapsid Chimeric monoclonal antibody at 1:2000 dilution.

Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell Lysate

Lane B: HEK293 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Human IgG Fc/HRP at 1/1000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Immunochemical analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Chimera Mab,then a HRP-conjugated second step antibody.
Immunofluorescence analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Chimera Mab,then a FITC-conjugated second step antibody.

SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb: Synonyms

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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