SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb
Cat: 40143-MM05TB
SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb General Information
Product name
SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb
Validated applications
WB,ELISA,IHC-P,FCM,ICC/IF
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
SARS Coronavirus Nucleocapsid
Has cross-reactivity in ELISA with
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Cat# 40588-V08B)
Has cross-reactivity in ELISA with
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Cat# 40588-V08B)
Immunogen
Recombinant SARS-CoV Nucleoprotein / NP Protein (Catalog#40143-V08B)
Preparation
It is a chimeric monoclonal antibody combining the human IgG1 Fc molecule with mouse scFv, and the variable region was from mouse monoclonal antibody (Cat#40143-MM05). The chimeric monoclonal antibody was produced using recombinant antibody technology.
Source
Monoclonal Mouse scFv / human IgG1 Fc Clone #05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:2000-1:5000 |
| ELISA | 1:1000-1:2000 |
| IHC-P | 1:100-1:500 |
| ICC-IF | 1:20-1:100 |
| FCM | 1:25-1:100 |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb Images
Flow cytometric analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Nucleocapsid Chimeric MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-SARS-CoV / SARS-CoV-2 Nucleocapsid Chimeric monoclonal antibody at 1:2000 dilution.
Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell Lysate
Lane B: HEK293 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Secondary
Goat Anti-Human IgG Fc/HRP at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Immunochemical analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Chimera Mab,then a HRP-conjugated second step antibody.
Immunofluorescence analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Chimera Mab,then a FITC-conjugated second step antibody.
SARS-CoV / SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb: Synonyms
Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody
Coronavirus Nucleocapsid Background Information
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
- 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
- Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
- Li W. et al., 2005, Science. 309 (5742): 1864-8.
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