MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified

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MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified General Information

Product name
MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified
Validated applications
WB,ELISA
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
MERS-CoV Coronavirus Nucleocapsid
Immunogen
Recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (Catalog#40068-V08B)
Preparation
Produced in rabbits immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP (Catalog#40068-V08B; AFS88943.1; Met 1-Asp413). Nucleoprotein specific IgG was purified by MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified Validated Applications

Application Dilution
WB 1:1000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified Images

Anti-Novel coronavirus (HCoV-EMC/2012) Nucleoprotein / NP rabbit polyclonal antibody at 1:1000 dilution

Sample:Novel coronavirus (HCoV-EMC/2012) Nucleoprotein / NP

Lane A: 10ng

Lane B: 5ng

Lane C: 2ng

Secondary

Goat anti Rabbit IgG (H+L)/HRP at 0.4ug/ml..

Performed under reducing conditions.

Citation List

MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified: Synonyms

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
  • Complement Receptor C5aR1 Inhibition Reduces Pyroptosis in hDPP4-Transgenic Mice Infected with MERS-CoV
    Author
    Jiang, Y;Li, J;Teng, Y;Sun, H;Tian, G;He, L;Li, P;Chen, Y;Guo, Y;Li, J;Zhao, G;Zhou, Y;Sun, S;
    Year
    2019
    Journal
    Viruses
    Application
    WB
  • Structure-Based Stabilization of Non-native Protein-Protein Interactions of Coronavirus Nucleocapsid Proteins in Antiviral Drug Design
    Author
    Lin, SM;Lin, SC;Hsu, JN;Chang, CK;Chien, CM;Wang, YS;Wu, HY;Jeng, US;Kehn-Hall, K;Hou, MH;
    Year
    2020
    Journal
    J. Med. Chem.
    Application
    IF

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