MERS-CoV Nucleocapsid Antibody, Mouse MAb

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MERS-CoV Nucleocapsid Antibody, Mouse MAb (Mouse Monoclonal antibody) General Information

Product name
MERS-CoV Nucleocapsid Antibody, Mouse MAb
Validated applications
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
MERS-CoV Coronavirus Nucleocapsid
Immunogen
Recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (Catalog#40068-V08B)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP (Catalog#40068-V08B; AFS88943.1; Met 1-Asp413). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #10
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

MERS-CoV Nucleocapsid Antibody, Mouse MAb (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1:1000-1:5000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

MERS-CoV Nucleocapsid Antibody, Mouse MAb (Mouse Monoclonal antibody) Images

Anti-MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP mouse monoclonal antibody at 1:1000

Sample:MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP

Lane A: 20ng

Lane B: 5ng

Lane C: 2ng

Secondary

Rabbit Anti-Mouse IgGF (ab)2/HRP at 1/10000 dilution.

Performed under reducing conditions.

MERS-CoV Nucleocapsid Antibody, Mouse MAb: Synonyms

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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