Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb

RRID Number: AB_2860110
EliteRmab®, manufactured by recombinant technology

Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb General Information

Product name
Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb
Validated applications
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
CMV CMV Glycoprotein B
Recombinant Human CMV gB / Glycoprotein B protein (Catalog#10202-V08H1)
This antibody was obtained from a rabbit immunized with purified, recombinant Human CMV gB (Catalog#10202-V08H1; AAA45920.1) extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907).
Monoclonal Rabbit IgG Clone #38
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb Validated Applications

Application Dilution
WB his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect Human CMV gB in WB. Using a DAB detection system, the detection limit for Human CMV gB is approximately 0.125 ng/lane under non-reducing conditions and 100 ng/lane under reducing conditions.
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

CMV Glycoprotein B Background Information

Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
  • Lopper M,et al. (2002). Disulfide bond configuration of human cytomegalovirus glycoprotein B. J Virol. 76(12): 6073-82.
  • Isaacson MK, et al. (2009) Human cytomegalovirus glycoprotein B is required for virus entry and cell-to-cell spread but not for virion attachment, assembly, or egress. J Virol. 83(8): 3891-903.
  • Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells
    Smargiasso, N;Nader, J;Rioux, S;Mazzucchelli, G;Boutry, M;De Pauw, E;Chaumont, F;Navarre, C;
    Int J Mol Sci

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