Human CD3 (humanized OKT3) Antibody General Information
Human CD3 (humanized OKT3) Antibody
Reacts with: Human
Human CD3 epsilon/CD3e
Human CD3 (humanized OKT3) Antibody is a humanized antibody of Orthoclone OKT3. Orthoclone OKT3 is an immunosuppressant drug given to reduce acute rejection in patients with organ transplants. It is a monoclonal antibody targeted at the CD3 receptor, a membrane protein on the surface of T cells. It was the first monoclonal antibody to be approved for clinical use in humans.
Human IgG4 Clone #H001
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
< 0.5 EU/mg
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Human CD3 (humanized OKT3) Antibody Validated Applications
Agonist Activity (Func/A)：
Agonist Activity – Measured by its ability to induce proliferation of human T cells. The ED50 for this effect is typically 2-8 ug/mL.
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Human CD3 (humanized OKT3) Antibody Images
The staining effect of Human CD3 (humanized OKT3) antibody to CD3 of Human Peripheral Blood Lymphocytes by Flow cytometric analysis. A: Lymphocytes were stained with PE-conjugated human CD3 (humanized OKT3) antibody. B: The binding effect of PE-conjugated humanized OKT3 antibody(Red histograms) in the lymphocytes can be blocked by pre-incubation of the CD3 antibody (Miltenyi Biotec, 130-093-387) for 20min (blue histograms).
CD3+ T cell staining of lymphocytes using Human CD3 (humanized OKT3) antibody by Flow cytometric analysis. A: Lymphocytes were stained with both PE-conjugated CD3(humanized OKT3) antibody and OKT3-FITC-eBioscience (eBioscience, 11-0037-41). B: Lymphocytes were stained with PE-conjugated humanized OKT3 antibody and CD19-APC (BD, 555415).
Effect of Human CD3 (humanized OKT3) antibody on the proliferation and IFN-gamma secretion of CD4+ T cell. The isolated CD4+ T cells(1e5/well) were cultured for 4 days in the 96-well cell plates coated with serial dilutions of CD3 (humanized OKT3) antibody. A. The cell proliferation level was detected using MTT detection; B. IFN-gamma secretion in the medium was measured by IFN-gamma Quantitative ELISA Kit.
T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.
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