Anti-S100A4 Antibody

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Anti-S100A4 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-S100A4 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Mouse
Specificity
Mouse S100A4
Immunogen
Recombinant Mouse S100A4 protein (Catalog#50484-M07E)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse S100A4 (rM S100A4 ; Catalog#50484-M07E; NP_035441.1; Met1-Lys101). S100A4 specific IgG was purified by Mouse S100A4 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-S100A4 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-S100A4 Antibody (Rabbit Polyclonal antibody) Images

Anti-S100A4 rabbit polyclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lane B: NIH-3T3 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:12 kDa

Observed band size:10 kDa

(We are unsure as to the identity of these extra bands.)

Anti-S100A4 Antibody: Alternative Names

Anti-18A2 Antibody; Anti-42a Antibody; Anti-Capl Antibody; Anti-FSp1 Antibody; Anti-metastasin Antibody; Anti-Mts1 Antibody; Anti-PeL98 Antibody; Anti-pk9a Antibody

S100A4 Background Information

S1A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S1 proteins containing two EF-hand calcium-binding motifs. In humans at least 2 S1 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S1A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S1A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin β, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S1A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.
Full Name
S100 calcium binding protein A4
References
  • Ambartsumian N. et al., 1995, Gene. 159: 125-30.
  • Marenholz I. et al., 2004, Biochem Biophys Res Commun. 322: 1111-22.
  • Helfman DM. et al., 2005, Br J Cancer. 92: 1955-8.
  • Saleem M. et al., 2006, Proc Natl Acad Sci. 103: 14825-30.
  • Boye K. et al., 2008, Int J Cancer. 123: 1301-10.
  • Garrett SC. et al., 2006, J Biol Chem. 281: 677-80.
  • Kriajevska M. et al., 2002, J Biol Chem. 277: 5299-335.
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