PTP alpha / PTPRA Protein, Human, Recombinant (aa 174-793, His & GST Tag)

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PTP alpha / PTPRA Protein, Human, Recombinant (aa 174-793, His & GST Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. Measured by its ability to bind biotinylated recombinant mouse SRC in a functional ELISA.
2. Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >40000 pmol/min/μg.
Protein Construction
A DNA sequence encoding the human PTPRA isoform 2 (P18433-2) cytoplasmic domain (Ala 174-Lys 793) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human PTPRA (aa 174-793)/GST chimera consists of 857 amino acids and has a calculated molecular mass of 99 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 20% gly, 3mM DTT
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Liquid. It is shipped out with blue ice.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PTP alpha / PTPRA Protein, Human, Recombinant (aa 174-793, His & GST Tag): Images

PTP alpha / PTPRA Protein, Human, Recombinant (aa 174-793, His & GST Tag): Alternative Names

HEPTP Protein, Human; HLPR Protein, Human; HPTPA Protein, Human; HPTPalpha Protein, Human; LRP Protein, Human; PTPA Protein, Human; PTPRL2 Protein, Human; R-PTP-alpha Protein, Human; RPTPA Protein, Human

PTP alpha / PTPRA Background Information

PTPRA is reported to be involved in cancer development and progression through activating the Src family kinase (SFK) signaling pathways.The higher PTPRA level was associated with worse prognosis of SCC patients and PTPRA could promote the cell cycle progression through stimulating the c-Src signaling pathways. The PTPRA gene, which encodes the protein RPTP-alpha, is critical to neurodevelopment. Previous linkage studies, genome-wide association studies, controlled expression analyses and animal models support an association with both schizophrenia and autism spectrum disorders, both of which share a substantial portion of genetic risks.
Full Name
protein tyrosine phosphatase, receptor type, A
References
  • Kaplan R, et al. (1990) Cloning of three human tyrosine phosphatases reveals a multigene family of receptor-linked protein-tyrosine-phosphatases expressed in brain. Proc Natl Acad Sci U S A. 87 (18): 7000-4.
  • Hertog JD, et al. (1996) Tight association of GRB2 with receptor protein-tyrosine phosphatase alpha is mediated by the SH2 and C-terminal SH3 domains. EMBO J. 15 (12): 3016-27.
  • Ye H, et al. (2011) Receptor-like protein-tyrosine phosphatase a enhances cell surface expression of neural adhesion molecule NB-3. J Biol Chem. 286 (29): 26071-80.
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