PD-L1 ELISA Kit, Human

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PD-L1 ELISA Kit, Human: General Information

Product name
PD-L1 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant,Plasma
Sensitivity
5.56 pg/mL
Assay range
10.94-700 pg/mL
Specificity
Recognizes both recombinant and natural Human PD-L1
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human PD-L1 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 117.17 256.52 517.06
SD 7.97 8.43 13.93
CV(%) 6.8% 3.3% 2.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 127.83 266.11 532.38
SD 9.23 10.63 18.89
CV(%) 7.2% 4.0% 3.5%
Recovery
The recovery of Human CD274 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
91
Range
77-109%
Sample
supernatant (n=3)
Average % Recovery
85
Range
70-96%
Sample
plasma (n=3)
Average % Recovery
84
Range
82-87%
Linearity
Serum
  Recovery of detected
1:2 100%
1:4 114%
1:8 109%
1:16 114%
Supernatant
  Recovery of detected
1:2 100%
1:4 107%
1:8 107%
1:16 105%
Plasma
  Recovery of detected
1:2 100%
1:4 91%
1:8 90%
1:16 87%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This PD-L1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human PD-L1 protein in Serum,Cell supernatant,Plasma . It contains recombinant Human PD-L1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

PD-L1 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural human PD-L1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

PD-L1 ELISA Kit, Human: Alternative Names

B7-H ELISA Kit, Human; B7-H1 ELISA Kit, Human; B7H1 ELISA Kit, Human; PD-L1 ELISA Kit, Human; PDCD1L1 ELISA Kit, Human; PDCD1LG1 ELISA Kit, Human; PDL1 ELISA Kit, Human

PD-L1 Background Information

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
Full Name
CD274 molecule
References
  • Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.
  • Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.
  • Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.
  • Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.
  • Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.
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