Anti-Neuropilin-1 Antibody

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Anti-Neuropilin-1 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-Neuropilin-1 Antibody
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human Neuropilin-1
Immunogen
Recombinant Human Neuropilin-1 / NRP1 / CD304 protein (Catalog#10011-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Neuropilin-1 / NRP1 / CD304 (rh Neuropilin-1 / NRP1 / CD304; Catalog#10011-H08H; NP_001019799.1; Met1-Lys644). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #01
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Neuropilin-1 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Neuropilin-1 Antibody (Mouse Monoclonal antibody) Images

Flow cytometric analysis of human NRP1(CD304) expression on HUVEC cells. Cells were stained with purified anti-Human NRP1, then a PE-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.

Anti-Neuropilin-1 Antibody: Alternative Names

Anti-BDCA4 Antibody; Anti-CD304 Antibody; Anti-Neuropilin-1 Antibody; Anti-NP1 Antibody; Anti-NRP Antibody; Anti-VEGF165R Antibody

Neuropilin-1 Background Information

Neuropilin is a type I transmembrane protein and the molecular mass is 12 kDa. Two homologues, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.
Full Name
neuropilin 1
References
  • Nakamura F, et al. (2002) Structural and functional relation of neuropilins. Adv Exp Med Biol. 515: 55-69.
  • Romeo PH, et al. (2002) Neuropilin-1 in the immune system. Adv Exp Med Biol. 515: 49-54.
  • Klagsbrun M, et al. (2002) The role of neuropilin in vascular and tumor biology. Adv Exp Med Biol. 515: 33-48.
  • Staton CA, et al. (2007) Neuropilins in physiological and pathological angiogenesis. J Pathol. 212(3): 237-48.
  • Bagri A, et al. (2009) Neuropilins in tumor biology. Clin Cancer Res. 15(6): 1860-4.
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