Junctional Adhesion Molecule B / JAM2 Protein, Mouse, Recombinant (Fc Tag)

1/1
Price:
Size:
Number:

Junctional Adhesion Molecule B / JAM2 Protein, Mouse, Recombinant (Fc Tag): Product Information

Purity
> 70 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by the ability of the immobilized protein to support the adhesion of Jurkat human leukemic T cells . When 8 x 10E4 cells/well are added to JAM2-coated plates (0.2 μg/ml and 100 μl/well), approximately 40-55% will adhere specifically after 60 minutes at 37℃.
Protein Construction
A DNA sequence encoding the mouse JAM-2 (NP_076333.3) extracellular domain (Met 1-Asn 236) was fused with the Fc region of human IgG1 at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Phe 29
Molecule Mass
The secreted recombinant mouse JAM-2/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 449 amino acids and has a predicted molecular mass of 50.3 kDa. rm JAM-2/Fc monomer migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Junctional Adhesion Molecule B / JAM2 Protein, Mouse, Recombinant (Fc Tag): Images

Junctional Adhesion Molecule B / JAM2 Protein, Mouse, Recombinant (Fc Tag): Alternative Names

1110002N23Rik Protein, Mouse; 2410030G21Rik Protein, Mouse; 2410167M24Rik Protein, Mouse; JAM-2 Protein, Mouse; JAM-B Protein, Mouse; Jcam2 Protein, Mouse; VE-JAM Protein, Mouse

Junctional Adhesion Molecule B / JAM2 Background Information

Junctional adhesion molecule B (JAM-B), also known as Junctional adhesion molecule 2 (JAM2), Vascular endothelial junction-associated molecule (VE-JAM), and CD322, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. It is prominently expressed on high endothelial venules. expression to be restricted to the high endothelial venule of tonsil and lymph nodes. The localization to the endothelium of arterioles in and around inflammatory and tumor foci. JAM-B can function as an adhesive ligand for the T cell line J45 and can interact with GM-CSF/IL-4-derived peripheral blood dendritic cells, circulating CD56(+) NK cells, circulating CD56(+)CD3(+) NK/T cells, and circulating CD56(+)CD3(+)CD8(+) cytolytic T cells. JAM-2 is expressed on high endothelial venules (HEVs) in human tonsil and on a subset of human leukocytes, suggesting that JAM-2 plays a central role in the regulation of transendothelial migration. It binds to very late activation antigen (VLA)-4, a leucocyte integrin that contributes to rolling and firm adhesion of lymphocytes to endothelial cells through binding to vascular cell adhesion molecule (VCAM)-1. JAM-B appears to contribute to leucocyte extravasation by facilitating not only transmigration but also rolling and adhesion. JAM-B acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs.
Full Name
junctional adhesion molecule 2
References
  • Johnson-Lger CA, et al. (2002) Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration. Blood. 2100(7): 2479-86.
  • Liang TW, et al. (2002) Vascular endothelial-junctional adhesion molecule (VE-JAM)/JAM 2 interacts with T, NK, and dendritic cells through JAM 3. J Immunol. 168(4): 1618-26.
  • Ludwig RJ, et al. (2009) Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin. Immunology. 128(2): 196-205.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait