IL-29 ELISA Kit, Human

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IL-29 ELISA Kit, Human: General Information

Product name
IL-29 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
1.44 pg/mL
Assay range
7.03-450 pg/mL
Specificity
Recognizes both recombinant and natural Human IL-29
Interference
Preparations of human IL28A、IL28B at 10ng/mL in a mid-range recombinant human IL29 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 80.00 162.00 295.00
SD 2.37 5.50 9.37
CV(%) 3.0% 3.4% 3.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 93.00 191.00 337.00
SD 2.20 16.31 17.71
CV(%) 2.4% 8.5% 5.3%
Recovery
The recovery of Human IFNL1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
80
Range
73 -92%
Linearity
Supernatant
  Recovery of detected
1:4 103%
1:8 92%
1:16 70%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-29 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-29 protein in Cell supernatant . It contains recombinant Human IL-29, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL-29 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL29. The factors listed below were prepared at 10 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL-29 ELISA Kit, Human: Alternative Names

IFNL1 ELISA Kit, Human; IL-29 ELISA Kit, Human; IL29 ELISA Kit, Human

IL-29 Background Information

Interleukin-29(IL-29), also known as cytokine Zcyto21, Interferon lambda-1, IFN-lambda-1, and IFNL1, is a secreted protein which belongs to the IL-28 / IL-29 family. IL-29 is a cytokine with immunomodulatory activity. IL-29 is highly similar in amino acid sequence to the IL-28. IL-28 and IL-29 are induced by viral infection and showed antiviral activity. IL-28 and IL-29 interacted with a heterodimeric class II cytokine receptor that consisted of IL-1 receptor beta (IL-1Rbeta) and an orphan class II receptor chain, designated IL-28Ralpha. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. IL-29 may play a role in antiviral immunity. IL-29 up-regulates MHC class I antigen expression. It is a Ligand for the heterodimeric class II cytokine receptor composed of IL1RB and IL28RA. The ligand / receptor complex seems to signal through the Jak-STAT pathway.
Full Name
interferon, lambda 1
References
  • Li MC, et al. (2006) Liposome-mediated IL-28 and IL-29 expression in A549 cells and anti-viral effect of IL-28 and IL-29 on WISH cells. Acta Pharmacol Sin. 27(4): 453-9.
  • Wang J, et al. (2009) Differentiated human alveolar type II cells secrete antiviral IL-29 (IFN-lambda 1) in response to influenza A infection. J Immunol. 182(3): 1296-304.
  • Jordan WJ, et al. (2007) Modulation of the human cytokine response by interferon lambda-1 (IFN-lambda1/IL-29). Genes Immun. 8(1): 13-20.
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