HIV-1 gp120 Protein (group M, subtype CRF07_BC) (His Tag)

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HIV-1 gp120 Protein (group M, subtype CRF07_BC) (His Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the HIV-1 envelope glycoprotein gp160 (AFM77980.1) extracellular domain (Ala 29-Glu 503), termed as gp120, was fused with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
HIV
Predicted N Terminal
Ala 29
Molecule Mass
The recombinant HIV-1 gp120 consists of 486 amino acids after removal of the signal peptide and has a predicted molecular mass of 54.6 kDa. The apparent molecular mass of the recombinant protein is approximately 80-100 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

HIV-1 gp120 Protein (group M, subtype CRF07_BC) (His Tag): Images

HIV gp120 Background Information

The HIV-1 gp120 envelope protein, a glycoprotein that is part of the outer layer of the virus, which is an essential component in the multi-tiered viral entry process. It presents itself as viral membrane spikes consisting of 3 molecules of gp120 linked together and anchored to the membrane by gp41 protein. Gp120 is essential for viral infection as it facilitates HIV entry into the host cell and this is its best-known and most researched role in HIV infection. However, it is becoming increasingly evident that gp12 might also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus. The surface protein gp120 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. Gp120 binding to its receptor CD4 and co-receptor, CXCR4 or CCR5 is required for fusion of viral and cellular membranes. Several mechanisms might be involved in this process of which gp120 binding to the CD4 receptor of T cells is the best known and most important interaction as it facilitates viral entry into the CD4+ cells and their depletion, a hallmark of the HIV infection. Gp120 is shed from the viral membrane and accumulates in lymphoid tissues in significant amounts. Despite the overall genetic heterogeneity of the gp120 glycoprotein, the conserved CD4 binding site provides an attractive antiviral target. Interaction between gp120 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS.
References
  • Kadow J, et al. (2006) Small-molecule HIV-1 gp120 inhibitors to prevent HIV-1 entry: an emerging opportunity for drug development. Curr Opin Investig Drugs. 7(8): 721-6.
  • Stevceva L, et al. (2007) Immune responses to HIV Gp120 that facilitate viral escape. Curr HIV Res. 5(1): 47-54.
  • Yoon V, et al. (2010) The GP120 molecule of HIV-1 and its interaction with T cells. Curr Med Chem. 17(8): 741-9.
  • Glycan analysis by isobaric aldehyde reactive tags and mass spectrometry
    Author
    Yang S, Yuan W, Yang W, Zhou J, Harlan R, Edwards J, Li S, Zhang H.
    Year
    2013
    Journal
    Anal. Chem.
    Application
    N-Glycans Isolation
  • Monovalent mannose-based DC-SIGN antagonists: targeting the hydrophobic groove of the receptor
    Author
    Tomašić, T;Hajšek, D;Švajger, U;Luzar, J;Obermajer, N;Petit-Haertlein, I;Fieschi, F;Anderluh, M;
    Year
    2014
    Journal
    Eur J Med Chem
    Application
    Competitive gp120-displacement assay
  • Pharmacokinetics and Preliminary Safety of Pod-Intravaginal Rings Delivering the Monoclonal Antibody VRC01-N for HIV Prophylaxis in a Macaque Model
    Author
    Zhao, C;Gunawardana, M;Villinger, F;Baum, MM;Remedios-Chan, M;Moench, TR;Zeitlin, L;Whaley, KJ;Bohorov, O;Smith, TJ;Anderson, DJ;Moss, JA;
    Year
    2017
    Journal
    Antimicrob. Agents Chemother.
    Application
    ELISA(as coating antigen)
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