L1CAM ELISA Kit, Human

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L1CAM ELISA Kit, Human: General Information

Product name
L1CAM ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
8.31 pg/mL
Assay range
39.06-2500 pg/mL
Specificity
Recognizes both recombinant and natural Human L1CAM
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 383.50 726.69 1,447.90
SD 20.08 41.19 48.79
CV(%) 5.2% 5.7% 3.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 393.77 792.05 1,524.00
SD 17.77 74.35 109.17
CV(%) 4.5% 9.4% 7.2%
Recovery
The recovery of Human L1CAM spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
91
Range
81-108%
Linearity
Serum
  Recovery of detected
1:2 94%
1:4 100%
1:8 100%
1:16 110%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This L1CAM ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human L1CAM protein in Serum . It contains recombinant Human L1CAM, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

L1CAM ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD171. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

L1CAM ELISA Kit, Human: Alternative Names

CAML1 ELISA Kit, Human; CD171 ELISA Kit, Human; HSAS ELISA Kit, Human; HSAS1 ELISA Kit, Human; MASA ELISA Kit, Human; MIC5 ELISA Kit, Human; N-CAM-L1 ELISA Kit, Human; N-CAML1 ELISA Kit, Human; NCAM-L1 ELISA Kit, Human; S10 ELISA Kit, Human; SPG1 ELISA Kit, Human

L1CAM Background Information

L1 cell adhesion molecule (L1CAM), also designated as CD171, is a cell adhesion receptor of the immunoglobulin superfamily, known for its roles in nerve cell function. While originally believed to be present only in brain cells, in recent years L1-CAM has been detected in other tissues, and in a variety of cancer cells, including some common types of human cancer. L1CAM interacts with a variety of ligands including axonin-1, CD9, neurocan and intergrins, and it has been revealed that the RGD motif in the sixth Ig domain of L1CAM is a binding site for integrins, thus important for nuclear signaling. Disruption of L1CAM function causes three X-linked neurological syndromes, i.e. hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait and adducted thumbs) and spastic paraplegia syndrome. Overexpression of L1CAM in normal and cancer cells increased motility, enhanced growth rate and promoted cell transformation and tumorigenicity. Recent work has identified L1CAM (CD171) as a novel marker for human carcinoma progression, and a candidate for anti-cancer therapy.
Full Name
L1 cell adhesion molecule
References
  • Meier F, et al. (2006) The adhesion molecule L1 (CD171) promotes melanoma progression. Int J Cancer. 119(3): 549-55.
  • Gavert N, et al. (2008) L1-CAM in cancerous tissues. Expert Opin Biol Ther. 8(11): 1749-57.
  • Issa Y, et al. (2009) Enhanced L1CAM expression on pancreatic tumor endothelium mediates selective tumor cell transmigration. J Mol Med. 87(1): 99-112.
  • Weidle UH, et al. (2009) L1-CAM as a target for treatment of cancer with monoclonal antibodies. Anticancer Res. 29(12): 4919-31.
  • Raveh S, et al. (2009) L1 cell adhesion molecule (L1CAM) in invasive tumors. Cancer Lett. 282(2): 137-45.
  • Wolterink S, et al. (2010) Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer Res. 70(6): 2504-15.
  • Diagnostic and prognostic value of serum L1-cell adhesion molecule in esophageal squamous cell carcinoma
    Author
    Xu, YW;Hong, CQ;Wu, ZY;Peng, YH;Ran, LQ;Yang, SH;Huang, BS;Liang, XY;Chen, HL;Wu, JY;Xu, XE;Deng, JW;Zou, HY;Fang, WK;Li, EM;Xu, LY;Xie, JJ;
    Year
    2018
    Journal
    Clin Res Hepatol Gastroenterol
    Application
    ELISA (serum sample)
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