CALB1 was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate
Lane B:0.5 mg 293T Whole Cell Lysate4 µL anti-CALB1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.Primary antibody:
Anti-CALB1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 30 kDa
Observed band size: 30 kDa
Anti-CALB1 rabbit polyclonal antibody at 1:500 dilution.
Lane A: Mosue kidney tissue lysateLysates/proteins at 30 μg per lane.
Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:30 kDa
Observed band size:27 kDa