BDNF Protein, Mouse / Human / Rat / Cynomolgus / Canine , Recombinant

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BDNF Protein, Mouse / Human / Rat / Cynomolgus / Canine , Recombinant: Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. Measured by its ability to bind biotinylated human TrkB-His (Cat:10047-H08H) in functional ELISA.
2. Measured by its ability to bind Human TrkB-Fch (Cat:10047-H03H) in functional ELISA.
3. Measured by its ability to bind biotinylated mouse TrkB-His (Cat:50132-M08H) in functional ELISA.
Protein Construction
A DNA sequence encoding the mouse BDNF (P21237) (Met1-Arg249) was expressed and its mature form was purified. The mature form of human, mouse, rat, cynomolgus and canine BDNF sequences are identical.
Accession#
Expressed Host
CHO Stable Cells
Species
Mouse
Predicted N Terminal
His 131
Molecule Mass
The recombinant mature form of mouse BDNF consists of 119 amino acids and predicts a molecular mass of 13.5 KDa.
Formulation
Lyophilized from sterile PBS
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

BDNF Protein, Mouse / Human / Rat / Cynomolgus / Canine , Recombinant: Images

BDNF Background Information

BDNF is a member of the nerve growth factor family. It is highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. It also can be detected in heart, lung, skeletal muscle, testis, prostate and placenta. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. During development, BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. It participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. It functions as the major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Full Name
brain-derived neurotrophic factor
References
  • Zigova T, et al. (1998) Intraventricular administration of BDNF increases the number of newly generated neurons in the adult olfactory bulb. Mol Cell Neurosci. 11(4):234-45.
  • Acheson A, et al. (1995) A BDNF autocrine loop in adult sensory neurons prevents cell death. Nature 374(6521):450-3.
  • Bekinschtein P, et al. (2008) BDNF is essential to promote persistence of long-term memory storage. Proc Natl Acad Sci. 105(7):2711-6.
  • Development of an innervated tissue-engineered skin with human sensory neurons and Schwann cells differentiated from iPS cells
    Author
    Muller, Q;Beaudet, MJ;De Serres-Bérard, T;Bellenfant, S;Flacher, V;Berthod, F;
    Year
    2018
    Journal
    Acta Biomater
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