Anti-TUBB4A Antibody

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Anti-TUBB4A Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-TUBB4A Antibody
Validated applications
WB,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human TUBB4A
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human TUBB4A
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human TUBB4A, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-TUBB4A Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:2000-1:5000
ICC-IF 1:300-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-TUBB4A Antibody (Rabbit Polyclonal antibody) Images

Anti-TUBB4A rabbit polyclonal antibody at 1:5000 dilution

Lane A: HeLa Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: HEK293 Whole Cell Lysate

Lane D: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Immunofluorescence staining of TUBB4A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TUBB4A polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoskeleton.

Anti-TUBB4A Antibody: Alternative Names

Anti-beta-5 Antibody; Anti-DYT4 Antibody; Anti-HLD6 Antibody; Anti-TUBB4 Antibody; Anti-TUBB5 Antibody

TUBB4A Background Information

The tubulin beta-4A gene (TUBB4A) is associated with two different clinical conditions, dystonia type 4 (DYT4) and hypomyelination with atrophy of the basal ganglia and cerebellum (H-ABC). Dystonia-4 (DYT4) is another autosomal dominant dystonia that is characterized by onset in the second to third decade of progressive laryngeal dysphonia. DYT4 is associated with the mutation of the TUBB4A gene. DYT4-associated TUBB4A mutants themselves form aberrant tubulin networks and inhibit neuronal process growth. Mutations in TUBB4A, encoding the tubulin isoform tubulin beta class IVA (Tubb4a), result in the symptom complex of hypomyelination with atrophy of basal ganglia and cerebellum (H-ABC). Additionally, TUBB4A mutations are known to result in a broad phenotypic spectrum, ranging from primary dystonia (DYT4), isolated hypomyelination with spastic quadriplegia, and an infantile onset encephalopathy, suggesting multiple cell types may be involved.
Full Name
tubulin, beta 4A class IVa
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