Anti-PGK2 Antibody

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Anti-PGK2 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-PGK2 Antibody
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology)
Specificity
Human PGK2
Immunogen
A synthetic peptide corresponding to the center region of the Human PGK2
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PGK2, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-PGK2 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IP IP:1-5 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PGK2 Antibody (Rabbit Polyclonal antibody) Images

Anti-PGK2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: HeLa Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:45 kDa

Observed band size:45 kDa

PGK2 was immunoprecipitated using:

Lane A:0.5 mg HeLa Whole Cell Lysate

4 µL anti-PGK2 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-PGK2 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 45 kDa

Observed band size :45 kDa

Anti-PGK2 Antibody: Alternative Names

Anti-dJ417L20.2 Antibody; Anti-HEL-S-272 Antibody; Anti-PGKB Antibody; Anti-PGKPS Antibody

PGK2 Background Information

Full Name
phosphoglycerate kinase 2

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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