Anti-E2F4 Antibody

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Anti-E2F4 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-E2F4 Antibody
Validated applications
WB,ICC/IF
Species reactivity
Reacts with: Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Specificity
Human E2F4
Immunogen
A synthetic peptide corresponding to the center region of the Human E2F4
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human E2F4, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-E2F4 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ICC-IF 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-E2F4 Antibody (Rabbit Polyclonal antibody) Images

Anti-E2F4 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lane B: Raji Whole Cell Lysate

Lane C: HeLa Whole Cell Lysate

Lane D: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:44 kDa

Observed band size:53 kDa

Immunofluorescence staining of E2F4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human E2F4 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Anti-E2F4 Antibody: Alternative Names

Anti-E2F-4 Antibody; Anti-E2F4 Antibody

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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