Anti-14-3-3 sigma Antibody

Cat: 10838-T54

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Anti-14-3-3 sigma Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-14-3-3 sigma Antibody
Validated applications
WB,ELISA,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human 14-3-3 sigma
Immunogen
Recombinant Human 14-3-3 sigma/Stratifin/YWHAS Protein (Catalog#10838-HNCE)
Preparation
Produced in rabbits immunized with purified, recombinant Human 14-3-3 sigma/Stratifin/YWHAS (rh 14-3-3 sigma/Stratifin/YWHAS; Catalog#10838-HNCE; NP_006133.1; Met1-Ser248). 14-3-3 sigma/Stratifin/YWHAS specific IgG was purified by Human 14-3-3 sigma/Stratifin/YWHAS affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-14-3-3 sigma Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
ICC-IF 1:300-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-14-3-3 sigma Antibody (Rabbit Polyclonal antibody) Images

Human 14-3-3 sigma/Stratifin/YWHAS Western blot (WB) 17227

Anti-SFN rabbit polyclonal antibody at 1:500 dilution

Lane A: HeLa Whole Cell Lysate

Lane B: A549 Whole Cell Lysate

Lane C: PC12 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:27 kDa

Observed band size:33 kDa

(We are unsure as to the identity of these extra bands.)

Human 14-3-3 sigma/Stratifin/YWHAS Immunofluorescence(IF) 17228
Immunofluorescence staining of SFN in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SFN polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human 14-3-3 sigma/Stratifin/YWHAS Immunoprecipitation(IP) 17229

SFN was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg A549 Whole Cell Lysate

2 µL anti-SFN rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SFN rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 27 kDa

Observed band size: 32 kDa

Anti-14-3-3 sigma Antibody Alternative Names

Anti-YWHAS Antibody

14-3-3 sigma Background Information

14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

Full Name
stratifin
References
  • Zakut-Houri R., et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255.
  • Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385.
  • Harlow E., et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal ELISA 50576-RP01
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50576-RP02
    Anti-14-3-3 sigma Antibody Mouse Monoclonal FCM 10838-MM02
    Anti-14-3-3 sigma Antibody (PE) Mouse Monoclonal FCM 10838-MM02-P
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal ELISA 10838-RP01
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal ELISA 10838-RP02
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