Anti-14-3-3 epsilon Antibody

Cat: 10842-MM07

Contact us:

215-583-7898
order_us@sinobiological.com
Distributors

All 14-3-3 epsilon Reagents

CRO Services

Product recommended


All 14-3-3 epsilon Reagents

Anti-14-3-3 epsilon Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-14-3-3 epsilon Antibody
Applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human 14-3-3 epsilon
Immunogen
Recombinant Human 14-3-3 epsilon / YWHAE Protein (Catalog#10842-HNCE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 epsilon / YWHAE (rh 14-3-3 epsilon / YWHAE; Catalog#10842-HNCE; NP_006752.1; Met1-Gln255). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #07
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-14-3-3 epsilon Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-14-3-3 epsilon Antibody Alternative Names

Anti-14-3-3E Antibody;Anti-HEL2 Antibody;Anti-KCIP-1 Antibody;Anti-MDCR Antibody;Anti-MDS Antibody

14-3-3 epsilon Background Information

YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 1% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.
Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
References
  • Ikeda M, et al. (2008) Identification of YWHAE, a gene encoding 14-3-3epsilon, as a possible susceptibility gene for schizophrenia. Hum Mol Genet. 17(20): 3212-22.
  • Mignon-Ravix C, et al. (2010) Deletion of YWHAE in a patient with periventricular heterotopias and pronounced corpus callosum hypoplasia. J Med Genet. 47(2): 132-6.
  • Nagamani SC, et al. (2009) Microdeletions including YWHAE in the Miller-Dieker syndrome region on chromosome 17p13.3 result in facial dysmorphisms, growth restriction, and cognitive impairment. J Med Genet. 46(12): 825-33.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-14-3-3 epsilon Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF,IP 10842-T54
    info info
    添加购物车成功! 添加购物车失败!