pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human Vimentin Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||HG10028-ACR|
|Human Vimentin Gene ORF cDNA clone expression plasmid, N-GFPSpark tag||HG10028-ANG|
|Human Vimentin Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||HG10028-ANR|
|Human Vimentin Gene ORF cDNA clone expression plasmid, C-Flag tag||HG10028-M-F|
|Human Vimentin Gene ORF cDNA clone expression plasmid||HG10028-UT|
|Learn more about expression Vectors|
Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.