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Transient Transfection Service: Mammalian Cell Culture

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Transient Transfection Service Description

Transient transfection, by definition, is different from stable cell culture. In transient transfection, DNA plasmid containing the target gene of interest is transfected into the host cells, usually HEK293 cells or CHO cells, with an efficient transfection reagent that can transport a large number of DNA plasmid through the cell membrane and into the nucleus for gene expression. Because the DNA plasmid's integration efficiency is very low, the majority of the transfected DNA remains in the cells without integrating into the chromosome, hence gene expression quickly decreases as cells divide and DNA degrades. With an engineered cell line, the cells can maintain a certain number of DNA copies in the cells for a short period of time, up to 6-12 days. The advantage of transient transfection is that it totally skipped the need for lengthy and costly stable cell line development, and hence it is highly preferable for quick and high-throughput small scale protein and antibody production.

Sino Biological has developed and optimized HEK293 cells and CHO cells for transient transfection. It has also optimized the serum-free culture medium and cell culture process to increase the efficiency of transient transfection. Furthermore, Sino Biological has developed........ <Learn More>

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
- Antibody CRO Service Description
- Antibody Service Q&A
- Antibody /Mab Production CRO Service
- Antibody Purification Service
- Antibody Humanization Service / Services
- Custom Antibody Development Service / Services
Protein Production CRO Service
- Protein Prodution Service description
- Protein protuction Capacity decription
- Description of Protein Production Experience and Track Records
- Protein production technology platform description
- Custom Protein Production project-service model
- transient transfection protein production service
- Protein production service in CHO cell culture stable production
- Protein Production Service in Insect Cell /Baculovirus Expression
- Protein Production Service in Yeast Expression System
- Protein Production Service in E. coli. Cells
- Protein Refolding Service / Protein Production Service in E. coli.
- Poly His-tag Protein Production & Purification Service
- DYKDDDDK-Tag (FLAG-Tag) Protein Production & Purification Service
- Fc-fusion-tag / Fc-Chimeric Protein Production & Purification Service
- HA-tag Protein Production & Purification Service
- Myc-tag Protein Production & Purification Service
- GST-fusion-tag Protein Production & Purification Service
Transient Transfection Service: Mammalian Cell Culture
- Transient Transfection Service Description
- HEK293 Cell Transient Transfection Service
- CHO Cell Transient Transfection Service
- Transient Transfection Service for Antibody Production
- Transient Transfection Service for Protein Production
Molecular Biology CRO Service
- Molecular Biology Service Description
- Gene Cloning CRO Service
- Gene Expression Vector Construction CRO Service
- Gene Synthesis CRO Service
- Real-time PCR Service: Gene Expression Analysis by qPCR
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"