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Transient Transfection Service Description

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Transient Transfection Service Description

Transient transfection, by definition, is different from stable cell culture. In transient transfection, DNA plasmid containing the target gene of interest is transfected into the host cells, usually HEK293 cells or CHO cells, with an efficient transfection reagent that can transport a large number of DNA plasmid through the cell membrane and into the nucleus for gene expression. Because the DNA plasmid's integration efficiency is very low, the majority of the transfected DNA remains in the cells without integrating into the chromosome, hence gene expression quickly decreases as cells divide and DNA degrades. With an engineered cell line, the cells can maintain a certain number of DNA copies in the cells for a short period of time, up to 6-12 days. The advantage of transient transfection is that it totally skipped the need for lengthy and costly stable cell line development, and hence it is highly preferable for quick and high-throughput small scale protein and antibody production.

Sino Biological has developed and optimized HEK293 cells and CHO cells for transient transfection. It has also optimized the serum-free culture medium and cell culture process to increase the efficiency of transient transfection. Furthermore, Sino Biological has developed a highly efficient, room temperature stable, and cost-effective mammalian cell transfection reagent, Sinofection, that allows very efficient gene transfection, making it possible to produce large quantities of bulk protein and bulk antibodies efficiently and at affordable cost. Multiple top multi-national pharmaceutical and biotech companies have taken advantages of our cutting-edge transient transfection platform to support their internal antibody discovery and development projects. Starting in 2015, Sino Biological is offering this service to the life science community and biotech industry to accelerate basic research and antibody product discovery and

Antibody Production: Cutting Edge Technology Platform

With years of process development, Sino Biological has built one of the better antibody production platforms for rapid and high-throughput antibody production and large scale antibody bulk production. From gene sequence to 10 grams of purified antibodies, it only take just a few weeks to deliver the product to your door. The advanced technology allows significant timing-saving and cost-saving. Many large pharmaceutical companies have taken advantages of our platform to support their in-house antibody discovery and development programs. We are proud to achieve almost 100% success rate in producing high quality antibody products on-time for thousands of monoclonal antibodies over the past 7 years (2007-2014). Our track records have brought well-known pharmaceutical and biotech customers totally by word-of-mouth with any business development and market efforts.

How to Submit a Request for Transient Transfection Service?

If you are interested in using our transient transfection services to support your antibody discovery and development program, please contact us by leaving an on-line message or send us your detailed request to CRO-service@sinobiological.com. Based on the quantity and quality requirement of your request, a formal quote will be send back to you for your review, comment, and/or acceptance.