Transient Transfection Antibody Production: Super-scale (1g - 100 gram)
|All Antibody Services||All Protein Services||All Gene Services||All Biological Services|
|High-Throughput:||10-30 monoclonal antibodies at 1g-100 g scale per month
one of the largest pre-clinical antibody production capacities worldwide
|Host Cells:||HEK293 or CHO cell (please specify, cost for production in CHO cell is higher)
|Technology:||Recombinant technology, transient transfection, Serum-free cell culture
Stirred-tank cell culture bioreactors
|Purification:||Affinity protein A/G/L chromatography column purification
plus ion exchange , SEC chromatography polishing steps as needed
|Timeline:||6-8 weeks total from receiving gene sequence to antibody delivery
- 1-2 weeks for gene synthesis
- 1-2 weeks for vector construction and plasmid production
- 4-5 weeks for transient transfection, antibody purification, QC testing
|Deliverable:||1 gram – 100 gram purified antibodies (quantities to be specified by customer)
|Quality Specification:||>95% antibody purity by SDS page with standard order
- For GLP grade antibody production order, we can meet endotoxin level as low as
0.1 EU/mg and/or monomer purity as high as >99% if required. Specifications on
endotoxin level and monomer purity can affect cost and may add a couple of weeks
time for process development, polishing steps, and additional QC testing.
|Antibody Types:||human, mouse, rat, rabbit, canine, rhesus IgG antibodies
- We have experience in producing IgA, IgM, and IgE antibodies
|Applications:||in vitro testing, in vivo mouse, rat, dog, rabbit, and monkey studies
antibody developability study, formulation development and stability
|Quotation:||on-line request form or e-mail request to CROemail@example.com|
Features of Antibody Production Services at Sino Biological
|Cutting-Edge Technology||Unlimited Capacity||Unbelievable Cost-Saving|
Key Features of Our Super-scale Antibody Production Service:
•Record Speed: 6-8 weeks from gene to purified antibody
•High-quality: can meet GLP quality standard with high monomer purity and low endotoxin
•Highly reliable: almost 100% success rate for unmodified antibodies
•Totally risk-free : if we fail, you don't need to pay
Sino Biological's super-scale antibody production service can be a great resource to support your antibody's pre-clinical development. Our advanced transient transfection technology in either HEK293 cells or CHO cells allows you to obtain up to 100 grams of GLP grade antibodies in just a few weeks for your large animal studies before you have a chance to develop a stable CHO cells line, and hence can greatly accelerate your antibody development timeline. The highly efficient transient transfection process not only makes it possible to generate large quantities of antibodies quickly but also at an affordable cost.
Our track-records in the past seven years (2008-2014) on the successful production of over 10,000 monoclonal antibodies have attracted top-10 multi-national pharmaceutical customers as well as small biotech companies worldwide through word-of-mouth, as we have not done much business development or advertisement in the past. In 2015, we are starting to promote ...... <Learn More>
Q: What is the timeline?
A:The timeline for antibody production is 6-8 weeks.
Q: I want to send plasmids to Sino Biological. How do I do?
A: Please prepare plasmids according to the following protocol for keeping plasmid in FTA elute card (Whatman Cat. No.: WB120410, WB120401, WB120461) at room temperature.
HANDLING: Always wear gloves to avoid contamination of FTA Elute Cards.
1). Label the FTA Elute Card with the appropriate sample identification. Use one card for each biological sample.
2). Pipet the biological sample (40μl per 11 mm circle) onto the card in a concentric circular motion within the printed circle area slowly.
3). Samples applied to FTA Elute Cards are ready for immediate room temperature storage. Note: If samples are to be processed shortly after application on the FTA Elute Card, allow the sample to dry completely for at least 3 hours at room temperature prior to punching. To reduce drying time, spotted FTA Elute cards can be placed at 80° C for 15-20 minutes.
Q: What purity and concentration of the final product does Sino Biological usually provide?
A: The final purity and concentration differs from product to product according to client's final requirement.
...... < Learn More>