Goat Anti-Human IgG Secondary Antibody (HRP)

Cat: SSA002

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Overview
 

Description:

Goat polyclonal Secondary Antibody to Human IgG (HRP).

Host

Goat

Target species:

Human IgG

Specificity:

By ELISA this antibody reacts specifically with Human IgG.

 

Has cross-reactivity with rabbit IgG, rat IgG (>10%), horse IgG (3.5%), guinea pig IgG (3.6%), human IgM (2.2%)

Minimal cross-reactivity with mouse IgG, bovine IgG

Applications:

ICC, IHC-P, ELISA, WB

 
Properties

Form:

Liquid

Storage & Stability:

Protected from prolonged exposure to light. Store at +4℃ short term (1-2 weeks) (Preservative is recommended to avoid contamination). Upon delivery aliquot. Store at -20℃ to -80℃ for twelve months from date of receipt. Avoid repeated freeze-thaw cycles.

Constituents:

PBS

Purification notes:

Immunogen affinity purified

Formulation:

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

 
Applications

The application notes include recommended starting dilutions, optimal dilutions/concentrations should be determined by the end user.

 

ICC

Use at an assay dependent dilution

IHC-P

1/200 - 1/2000

ELISA

1/10000 - 1/100000 (Primary)

 WB

1/2000 - 1/25000

(Colormetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)

 

Other Proteins

info info
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