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ssPA / Stringent starvation protein A Antibody, Rabbit MAb

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E. coli SSPA Antibody Product Information
Immunogen:Recombinant E. coli ssPA / Stringent starvation protein A protein (Catalog#13098-E07E)
Clone ID:113
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant E. coli ssPA / Stringent starvation protein A (Catalog#13098-E07E; P0ACA3; Met1-Ser212).
E. coli SSPA Antibody Usage Guide
Specificity:E. coli ssPA / Stringent starvation protein A

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect E. coli ssPA / Stringent starvation protein A. The detection limit for E. coli ssPA / Stringent starvation protein A is approximately 0.00975 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other SSPA Antibody Products
ssPA/Stringent starvation protein A Background

Stringent starvation protein A (ssPA), a member of the GST superfamily, is an RNA polymerase-associated transcriptional activator for the lytic development of phage P1 and is essential for stationary phase-induced acid tolerance of E. coli. It indicate that the flexible regions are not critical for SspA function, whereas the surface pocket is important for both transcriptional activation of the phage P1 late promoter and acid resistance of E. coli. SspA is known to be implicated in survival during nutrient starvation and prolonged stationary phase. Recently, SspA was shown to play an important role in the stationary phase-induced stress response including acid tolerance by down-regulating the level of the global regulator H-NS, which negatively regulates multiple stress defense systems.

E. coli ssPA/Stringent starvation protein A References
  • Hansen AM, et al. (2003) Escherichia coli SspA is a transcription activator for bacteriophage P1 late genes. Mol Microbiol. 48(6): 1621-31.
  • Hommais F, et al. (2001) Large-scale monitoring of pleiotropic regulation of gene expression by the prokaryotic nucleoid-associated protein, H-NS. Mol Microbiol. 40(1): 20-36.
  • Atlung T, et al. (1997) H-NS: a modulator of environmentally regulated gene expression. Mol Microbiol. 24(1): 7-17.
  • Williams MD, et al. (1994) Starvation-induced expression of SspA and SspB: the effects of a null mutation in sspA on Escherichia coli protein synthesis and survival during growth and prolonged starvation. Mol Microbiol. 11(6): 1029-43.
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    Catalog: 13098-R113-100
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