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Single-Chain ScFv Antibody Fragment Production Service

All Antibody Services All Protein Services All Gene Services All Biological Services

ScFv Single Chain Antibody Fragment Production Service

Host Cells: E. coli., Yeast, or HEK293
Production: Recombinant technology
- E. coli. fermentation: soluble expression
- Yeast Fermentation: secrected protein expression
- Mammalian cell transient transfection
Purification: His-tag, FLAG-tag etc. Affinity chromatography column purification
Anion and Caton Exchange Chromatography
and Size Exclusion Chromatography Column (SEC) for polishing if needed
Purification Tags: 6-10 His tag, FLAG tag, SUMO-tag, etc. as chosen by customer
Timeline: 5-8 weeks total from receiving gene sequence to product delivery
- 1-2 weeks for gene synthesis
- 1-2 weeks for vector construction and plasmid production
- 3-4 weeks for production, purification, and QC testing
Deliverable: Purified single chain antibody ScFv (quantities to be specified by customer)
Quality Specification: as specified by cutomer (cost may be adjusted based on quality spec)
Antibody Types: human, mouse, rat, rabbit, canine, rhesus IgG antibodies
Applications: drug discovery: antibody antigen co-crystalization, antibody binding eptiope
Quotation: on-line request form or e-mail request to CRO-service@sinobiological.com

Track Records & Key Facts

  • • > 300 Staff
  • • > 80 Bioreactors
  • • > 30 Purification System
  • • > 10,000 Mab Production
  • • > 5000 Protein Bulks
  • • > 5000 antibody development
  • • 1-100mg Mab Production in 3-6 weeks
  • • 1-10 g Mab Production in 6-8 weeks
  • • Preferred CRO Partner for Multiple Top-10 Pharmas
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Features of Antibody Production Services at Sino Biological

  • Record Speed in Antibody Production
  • • From Mab Sequence to Purified Mab
  • • 1-100 mg Mab in 3-6 weeks
  • • 1-10 gram Mab in 6-8 weeks
  • GLP Grade Antibody Production
  • • High Monomer Purity: up to 99%
  • • Low Endotoxin Level: up to 0.05 EU/mg
  • Unmatched Experience in Antibody Production
  • • >10,000 Mab Production
  • • Almost 100% success rate
Cutting-Edge Technology Unlimited Capacity Unbelievable Cost-Saving

ScFv Single Chain Antibody Fragment Production Service Description

ScFv single chain antibody fragment expression and production is a routine work to support antibody discovery with antibody library. ScFv protein can be readily expressed in microbial expression system such as E. coli. It can also be expressed in mammalian cells through transient transfection. Sino Biological is capable of producing ScFv single chain antibody fragment using either production systems, depending on customer's preference.

A suitable purification tag is typically ...... <Learn More>

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
- Antibody CRO Service Description
- Antibody Service Q&A
- Antibody /Mab Production CRO Service
-- Antibody Production Service Description
-- Ab Production Service Q&A
-- Antibody /Mab Production Service: mini-scale (0.1-1 mg)
mini-scale Mab production description
mini-scale Mab production Q&A
-- Antibody /Mab Production Service: Small-scale (1-10 mg)
small-scale Mab production description
small scale Mab production Q&A
-- Antibody /Mab Production Service: Mid-scale (10-100 mg)
Mid-scale Mab production Description
Mid-scale Mab production Q&A
-- Antibody /Mab Production Service: Large-scale (100 mg - 1 gram)
Large-scale Mab production Description
Large scale Mab production Q&A
-- Transient Transfection Antibody Production: Super-scale (1g - 100 gram)
Super-scale Mab by Transient transfection Description
Super-scale Mab by Transient transfection Q&A
-- Stable CHO Antibody Production Service: Super-scale (1g - 100 gram)
Super Scale Mab by CHO Description
-- Single-Chain ScFv Antibody Fragment Production Service
ScFv antibody fragment Description
-- Antibody / Mab Fab production Service
Antibody Fab Production Service Description
-- Mouse Mab production CRO Service
Mouse Mab Production Description
mouse Mab production from hybridoma cell culture
recombinant mouse Mab production service
-- Rat antibody production CRO service
Description of Rat Monoclonal Antibody Production Service
Rat Mab production from hybridoma cell culture
Recombinant Rat Mab production service
-- Rabbit Mab production CRO Service
Rabbit Mab production service Description
Rabbit Antibody /Mab Production Service: Hybridoma Culture
Recombinant Rabbit Mab Production Service
- Antibody Purification Service
- Antibody Humanization Service / Services
- Custom Antibody Development Service / Services
Protein Production CRO Service
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"