FCGR4 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated

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FCGR4 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Biotin/Protein Ratio
0.5-1 as determined by the HABA assay.
Activity
Measured by its ability to bind human IgG1 (Fc) (Cat:10702-HNAC) in a functional ELISA.
Protein Construction
The amino acid sequence corronding to (Met 1-Gln 203) of mouse FCGR4 (NP_653142.2) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Accession#
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Glu 19
Molecule Mass
The recombinant mouse FCGR4 consists of 214 amino acids and has a predicted molecular mass of 24.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 35-40 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FCGR4 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Images

Measured by its ability to bind human IgG1 (Fc) (Cat:10702-HNAC) in a functional ELISA.

FCGR4 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Alternative Names

4833442P21Rik Protein, Mouse; CD16-2 Protein, Mouse; FcgammaRIV Protein, Mouse; Fcgr3a Protein, Mouse; FcgRIV Protein, Mouse; Fcrl3 Protein, Mouse

FCGR4 Background Information

Full Name
Fc receptor, IgG, low affinity IV
  • Conserved FcγR- glycan discriminates between fucosylated and afucosylated IgG in humans and mice.
    Author
    Dekkers, G;Bentlage, AEH;Plomp, R;Visser, R;Koeleman, CAM;Beentjes, A;Mok, JY;van Esch, WJE;Wuhrer, M;Rispens, T;Vidarsson, G;
    Year
    2017
    Journal
    Mol. Immunol.
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