Real-time PCR Service: Gene Expression Analysis by qPCR

Real-time Quantitative PCR(qPCR)

qPCR amplification schematic

qPCR Service Content

qualitative and quantitative gene expression analysis

Qualitative Analysis

— detect viruses; detect GMO; identify cultivar; Mutant genotyping

Identification of transgenic mice with human IL6 use SYBR Green qPCR

Relative Quantitative Analysis

— reference gene and target gene expression analysis

Reference gene and target gene expression analysis in samples

Absolute Quantitative Analysis

— absolute quantitative analysis of DNA(RNA) ; transgene copy determination; virus and pathogen quantification

Detect the copy number changes of nodavirus after it infect the HighFive cells

SNP Analysis

— human disease analysis; Individuation of medical treatment; HRM analysis

Human CA12 gene SNP analysis by HRM

SYBR Green and Taqman Probe qPCR

Quantitative real-time-PCR (qPCR) such as TaqMan and SYBR Green qPCR assays are widely used for gene expression analysis.The advantages and disadvantages of two methods see the table below.
Basic principle of SYBR Green dye method	Basic principle of TaqMan probe method

Gene Expression Analysis by qPCR Advantages

Sino biological provide you with SYBR Green dye method and TaqMan probe method for gene expression analysis according to your scientific research demand. Qualitative analysis, relative quantification or absolute quantification can be completed by real-time quantitative PCR.

1.Sino biological has more than 10,000 gene plasmids with high quality and authenticated primer pairs. The detection efficiency is improved greatly.

2. Sino biological also has a number of experienced technical personnels who design qPCR detection schemes and present experimental service.

qPCR Service Process

Taking an example of TaqMan probe method
1. Design and synthesize primers and probes
Customers provide gene information for us, primers and probes are designed by our own algorithm of the company.

2. Perform a real-time PCR experiment
A real-time PCR is performed after RNA in the samples provided by customers is extracted and purified. The examining report is presented.