Real-time PCR Service: Gene Expression Analysis by qPCR

Powerful qPCR Technology Platform: Unique Reagents and Products

qPCR Master Mix	10000+qPCR primers	10000+qPCR standards
			High specificity High sensitivity High amplification efficiency Wide linear range
Sensitive	Specific	Accurate

Real-time Quantitative PCR(qPCR)

A real-time quantitative polymerase chain reaction(qPCR) is a technique of molecular biology, that is used to amplify and simultaneously detect or quantify targeted DNA molecules. The procedure follows the general principle of PCR. During PCR reaction progress the accumulation of PCR products results in the enhancement of fluorescence signals as the cycle numbers increase. Thus PCR reaction progress is detected in "real time" by monitoring the fluorescence intensity.

qPCR amplification schematic

qPCR Service Content

qualitative and quantitative gene expression analysis

Qualitative Analysis
— detect viruses; detect GMO; identify cultivar; Mutant genotyping

Identification of transgenic mice with human IL6 use SYBR Green qPCR

Relative Quantitative Analysis
— reference gene and target gene expression analysis

Reference gene and target gene expression analysis in samples

Absolute Quantitative Analysis
— absolute quantitative analysis of DNA(RNA) ; transgene copy determination;
virus and pathogen quantification

Detect the copy number changes of nodavirus after it infect the HighFive cells

SNP Analysis
— human disease analysis; Individuation of medical treatment; HRM analysis

Human CA12 gene SNP analysis by HRM

SYBR Green and Taqman Probe qPCR

Quantitative real-time-PCR (qPCR) such as TaqMan and SYBR Green qPCR assays are widely used for gene expression analysis.The advantages and disadvantages of two methods see the table below.

Basic principle of SYBR Green dye method

Basic principle of TaqMan probe method

Gene Expression Analysis by qPCR Advantages

Sino biological provide you with SYBR Green dye method and TaqMan probe method for gene expression analysis according to your scientific research demand. Qualitative analysis, relative quantification or absolute quantification can be completed by real-time quantitative PCR.

1.Sino biological has more than 10,000 gene plasmids with high quality and authenticated primer pairs. The detection efficiency is improved greatly.

2. Sino biological also has a number of experienced technical personnels who design qPCR detection schemes and present experimental service.

qPCR Service Process

Taking an example of TaqMan probe method
1. Design and synthesize primers and probes
Customers provide gene information for us, primers and probes are designed by our own algorithm of the company.

2. Perform a real-time PCR experiment
A real-time PCR is performed after RNA in the samples provided by customers is extracted and purified. The examining report is presented.

Examples of qPCR Products

Example 1

The detection results of 45 genes amplified in cDNA of human tissues by SYBR Green qPCR method.

Figure 1. Amplification curves of 45 genes amplified in cDNA of human tissues

Figure 2. Melting curves of 45 genes amplified in cDNA of human tissues

Example 2

Quality report of one gene primer pairs (including standard curve).

Figure 3. Amplification curves of SDHA standard samples

Figure 4. Standard curve and amplification efficiency of SDHA

Example 3

qPCR primers of reference genes and standard samples synthesized in our company. Learn more qEASY qPCR primer pairs.

Product Description	Catalog#(PDF)	Price
Human ACTB qPCR primer pairs	HP100001	$79
Human B2M qPCR primer pairs	HP100002	$79
Human GAPDH qPCR primer pairs	HP100003	$79
Human GUSB qPCR primer pairs	HP100004	$79
Human HPRT1 qPCR primer pairs	HP100005	$79
Human HSP90AB1 qPCR primer pairs	HP100006	$79
Human LDHA qPCR primer pairs	HP100007	$79
Human NONO qPCR primer pairs	HP100008	$79
Human PGK1 qPCR primer pairs	HP100009	$79
Human PPIA qPCR primer pairs	HP100010	$79
Human PPIH qPCR primer pairs	HP100011	$79
Human RPLP0 qPCR primer pairs	HP100012	$79
Human RPLP1 qPCR primer pairs	HP100013	$79
Human SDHA qPCR primer pairs	HP100014	$79
Human TBP qPCR primer pairs	HP100015	$79
Human TFRC qPCR primer pairs	HP100016	$79

Figure 5. Quality verification of qPCR primers of part reference genes

The experiment using the cDNA of seven human cancer cells as templates and the corresponding qEASY qPCR primer pairs for SYBR Green quantitative detection.