|qualitative and quantitative gene expression analysis|
|— detect viruses; detect GMO; identify cultivar; Mutant genotyping|
|Identification of transgenic mice with human IL6 use SYBR Green qPCR|
|Relative Quantitative Analysis|
|— reference gene and target gene expression analysis|
|Reference gene and target gene expression analysis in samples|
|Absolute Quantitative Analysis|
|— absolute quantitative analysis of DNA(RNA) ; transgene copy determination;
virus and pathogen quantification
|Detect the copy number changes of nodavirus after it infect the HighFive cells|
|— human disease analysis; Individuation of medical treatment; HRM analysis|
|Human CA12 gene SNP analysis by HRM|
|Quantitative real-time-PCR (qPCR) such as TaqMan and SYBR Green qPCR assays are widely used for gene expression analysis.The advantages and disadvantages of two methods see the table below.|
|Basic principle of SYBR Green dye method||Basic principle of TaqMan probe method|
Sino biological provide you with SYBR Green dye method and TaqMan probe method for gene expression analysis according to your scientific research demand. Qualitative analysis, relative quantification or absolute quantification can be completed by real-time quantitative PCR.
1.Sino biological has more than 10,000 gene plasmids with high quality and authenticated primer pairs. The detection efficiency is improved greatly.
2. Sino biological also has a number of experienced technical personnels who design qPCR detection schemes and present experimental service.
Taking an example of TaqMan probe method
1. Design and synthesize primers and probes
Customers provide gene information for us, primers and probes are designed by our own algorithm of the company.
2. Perform a real-time PCR experiment
A real-time PCR is performed after RNA in the samples provided by customers is extracted and purified. The examining report is presented.
|The detection results of 45 genes amplified in cDNA of human tissues by SYBR Green qPCR method.|
|Figure 1. Amplification curves of 45 genes amplified in cDNA of human tissues||Figure 2. Melting curves of 45 genes amplified in cDNA of human tissues|
|Quality report of one gene primer pairs (including standard curve).|
|Figure 3. Amplification curves of SDHA standard samples||Figure 4. Standard curve and amplification efficiency of SDHA|
|qPCR primers of reference genes and standard samples synthesized in our company. Learn more qEASY qPCR primer pairs.|
|Figure 5. Quality verification of qPCR primers of part reference genes|
|The experiment using the cDNA of seven human cancer cells as templates and the corresponding qEASY qPCR primer pairs for SYBR Green quantitative detection.|
|The expression level of GAPDH is highest and SDHA is most stable.|