PCSK9 ELISA Kit, Rat

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PCSK9 ELISA Kit, Rat: General Information

Product name
PCSK9 ELISA Kit, Rat
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
4.45 pg/mL
Assay range
23.44-1500 pg/mL
Specificity
Recognizes both recombinant and natural Rat PCSK9
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 245.85 503.00 996.07
SD 6.08 18.05 18.44
CV(%) 2.5% 3.6% 1.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 261.28 530.35 1,039.16
SD 15.03 21.86 28.34
CV(%) 5.8% 4.1% 2.7%
Recovery
The recovery of Rat PCSK9 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
88.5163425175581
Range
81-94%
Linearity
Serum
  Recovery of detected
1:2 102%
1:4 100%
1:8 98%
1:16 92%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This PCSK9 ELISA Kit, Rat is an enzyme-linked immunosorbent assay for the quantitative measurement of Rat PCSK9 protein in Serum . It contains recombinant Rat PCSK9, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

PCSK9 ELISA Kit, Rat: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Rat PCSK9. The factors listed below were prepared at 75 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

PCSK9 Background Information

Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

References

Full Name
proprotein convertase subtilisin/kexin type 9
References
  • Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933.
  • Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498
  • Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73.
  • Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875.
  • Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
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