qEASY qPCR Primer Pairs are designed using SBI's proprietary primer design algorithm. They are used for SYBR Green dye-based real-time PCR and designed according to the conserved region of all variants of a specific gene. At least one primer crosses the junction of adjacent exons to avoid amplification of genomic DNA directly and effectively. Our primer pairs cover all genes from human, mouse, rat and can be widely applied to the quantitative analysis of gene expression. cDNA used as templates, a single, correct-size band is produced in SYBR Green dye-based PCR with each pair of primers. Therefore, our primers have the characteristics with high specificity, high amplification efficiency, wide linear range and uniform reaction conditions. Each package for a specific gene is supplied with a lyophilized mixture of forward and reverse primers that can be used directly in SYBR Green dye-based real-time PCR after they are dissolved into ultrapure water.
Species | Number of qPCR primer pairs | Size | Storage | Notes |
Human | 6000+ | 2nmol/tube | Lyophilized qPCR primer mix | Containing positive organization validation report |
Mouse | 4000+ | 2nmol/tube | Lyophilized qPCR primer mix | Containing positive organization validation report |
Rat | 2000+ | 2nmol/tube | Lyophilized qPCR primer mix | Containing positive organization validation report |
45 pairs of gene-specific qPCR primers as examples,positive tissue cDNA and gene standard validation:
Amplification curves: amplification tendency enhanced | Melting curves: high specificity |
Electrophoresis detection: high specificity | Standard curve: high sensitivity, detectable limit is less than 100 copies, high amplification efficiency, wide linear range |
45 pairs of gene-specific qPCR primers were experimentally validated by SYBR Green method using first-strand cDNA and ddH2O as template. There were no non-specific amplification and primer-dimers produced by melting curve analysis and agarose gel electrophoresis detection. We used a dilution series (10-fold dilutions) of a purified plasmid as templates to construct standard curves for primer by SYBR Green-based real-time RT-PCR. We drew the standard curve by SYBR Green method in the qPCR experiment. The result showed that the qPCR Primers detectable limit is less than 100 copies and the amplification efficiency is between 90% ~ 105%. a. Amplification curves of 45 primer pairs in cDNA. (cDNA and ddH2O as templates);b. Melting curves of 45 primer pairs corresponding with a; c. Agarose gel electrophoresis validation result corresponding with a, the odd number lane is cDNA template detection, the even number lane is the corresponding NTC (No Template Control) results; d. Statistical graph of amplification efficiency about 45 primer pairs with a dilution series plasmid as templates; e. Amplification curves of SDHA standard curve; f. Standard curve of SDHA, the amplification efficiency is 99.0%.
Gene | Catalog# | Product Description | Availability |
GAPDH | HP100003 | Human GAPDH qPCR primer pairs | In stock |
MERTK | HP100055 | Human MERTK qPCR primer pairs | In stock |
FGF21 | HP100794 | Human FGF21 qPCR primer pairs | In stock |
OR1D2 | HP104420 | Human OR1D2 qPCR primer pairs | In stock |
IL1B | HP100210 | Human IL1B qPCR primer pairs | In stock |
PPARG | HP101555 | Human PPARG qPCR primer pairs | In stock |
IL6 | HP100427 | Human IL6 qPCR primer pairs | In stock |
CD274 | MP200010 | Mouse CD274 qPCR primer pairs | In stock |
TNF | HP100592 | Human TNF qPCR primer pairs | In stock |
PFKFB3 | HP102593 | Human PFKFB3 qPCR primer pairs | In stock |
CD274 | HP100170 | Human CD274 qPCR primer pairs | In stock |
FAP | HP101274 | Human FAP qPCR primer pairs | In stock |