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Poly His-tag Protein Production & Purification Service

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Poly His-tag Protein Production & Purification Service

Full services: From gene cloning/synthesis to purified proteins
Host Cells: Choices of Expression Hosts:
Transient Transfection in HEK293 Cells
Transient Transfection in CHO cells
Stable expression in CHO cells
Yeast cell expression system
Insect cell/baculovirus expression system
E. coli. microbial expression system
His Tag Design: 6x Histidine up to 10x histidine tag at N- or C-terminus
Cleavage Site: Option to design a cleavage site between the tag and protein
Selection of Cleavage Protease Enzyme: Enterokinase (EK),
HRV-3C (human rhinovirus protease), TEV
Purification: Immobilized metal ion affinity chromatography (IMAC)
- Nickel column
- Copper column
Deliverable: Purified recombinant proteins (quantities to be specified by customer)
Quality Specification: typically >90% purity by SDS page
Typical Timeline: 8-12 weeks

Track Records & Key Facts

  • • > 300 Staff
  • • > 80 Bioreactors
  • • > 30 Purification System
  • • > 10,000 Mab Production
  • • > 5000 Protein Bulks
  • • > 5000 antibody development
  • • 1-100mg Mab Production in 3-6 weeks
  • • 1-10 g Mab Production in 6-8 weeks
  • • Preferred CRO Partner for Multiple Top-10 Pharmas
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Poly-his-tag Protein Expression, Production, and Purification Basics

What is a poly-his-tag?

A polyhistidine-tag is an amino acid motif consisting of six-ten histidine (His) residues, either fused at the N- or C-terminus of a protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag.

Why use a poly-his-tag for protein expression and production?

Adding a poly-his-tag to the N- or C-terminus of a protein allows rapid, simple, and efficient purification of ...... < Learn More>

Poly-His-tag Protein Production & Purification Service Description

Poly-histidine tag (his-tag) has been widely used in recombinant protein expression and production. The his-tag allows simple and efficient purification of the tagged protein using Immobilized metal (Nickel or Copper) ion affinity chromatography (IMAC). The his-tag also can be used for detection in western blot by using a his-tag-specific antibody. His-tag can be added to either C- or N-terminus of a protein for expression in virtually all expression systems.

In the past couple of years, Sino Biological has successfully produced many proteins with a his-tag and purified with IMAC columns. We are highly experienced in designing ...... < Learn More>

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
Protein Production CRO Service
- Protein Prodution Service description
- Protein protuction Capacity decription
- Description of Protein Production Experience and Track Records
- Protein production technology platform description
- Custom Protein Production project-service model
-- Custom Protein Production Project Description
-- Description of Risk-sharing Protein Production
-- Description of Risk-free Protein Production Project
- transient transfection protein production service
- Protein production service in CHO cell culture stable production
- Protein Production Service in Insect Cell /Baculovirus Expression
-- Description of Protein Production Service in Insect Cells
- Protein Production Service in Yeast Expression System
-- Description of Protein Production Service in Yeast
- Protein Production Service in E. coli. Cells
-- Description of Protein Production Service in E. coli.
- Protein Refolding Service / Protein Production Service in E. coli.
-- Description of Protein Refolding & Production Project
- Poly His-tag Protein Production & Purification Service
-- Description of Risk-free Protein Production Project
-- Poly-his-tag Protein Expression, Production, and Purification Basics
- DYKDDDDK-Tag (FLAG-Tag) Protein Production & Purification Service
-- DYKDDDK-tag (FLAG-tag) Protein Production Service Description
-- FLAG-tag Protein Expression, Production, and Purification Basics
- Fc-fusion-tag / Fc-Chimeric Protein Production & Purification Service
-- Description of Fc-tag Protein Production & Purification Service
-- IgG-Fc-tag Protein Expression, Production, and Purification Basics
- HA-tag Protein Production & Purification Service
-- HA-tag Protein Production & Purification Service Description
-- HA-tag Protein Expression, Production, and Purification Basics
- Myc-tag Protein Production & Purification Service
-- Myc-tag Protein Production & Purification Service Description
-- Myc-tag Protein Expression, Production, and Purification Basics
- GST-fusion-tag Protein Production & Purification Service
-- GST-tag Protein Production & Purification Service Description
-- GST-tag Protein Expression, Production, and Purification Basics
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"