|All Antibody Services||All Protein Services||All Gene Services||All Biological Services|
|Full services:||From gene cloning/synthesis to purified proteins
|Host Cells:||Choices of Expression Hosts:
Transient Transfection in HEK293 Cells
Transient Transfection in CHO cells
Stable expression in CHO cells
Yeast cell expression system
Insect cell/baculovirus expression system
E. coli. microbial expression system
|His Tag Design:||6x Histidine up to 10x histidine tag at N- or C-terminus
|Cleavage Site:||Option to design a cleavage site between the tag and protein
Selection of Cleavage Protease Enzyme: Enterokinase (EK),
HRV-3C (human rhinovirus protease), TEV
|Purification:||Immobilized metal ion affinity chromatography (IMAC)
- Nickel column
- Copper column
|Deliverable:||Purified recombinant proteins (quantities to be specified by customer)|
|Quality Specification:||typically >90% purity by SDS page
|Typical Timeline:||8-12 weeks|
What is a poly-his-tag?
A polyhistidine-tag is an amino acid motif consisting of six-ten histidine (His) residues, either fused at the N- or C-terminus of a protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag.
Why use a poly-his-tag for protein expression and production?
Adding a poly-his-tag to the N- or C-terminus of a protein allows rapid, simple, and efficient purification of ...... < Learn More>
Poly-histidine tag (his-tag) has been widely used in recombinant protein expression and production. The his-tag allows simple and efficient purification of the tagged protein using Immobilized metal (Nickel or Copper) ion affinity chromatography (IMAC). The his-tag also can be used for detection in western blot by using a his-tag-specific antibody. His-tag can be added to either C- or N-terminus of a protein for expression in virtually all expression systems.
In the past couple of years, Sino Biological has successfully produced many proteins with a his-tag and purified with IMAC columns. We are highly experienced in designing ...... < Learn More>