Anti-Peroxiredoxin 1 Antibody

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Anti-Peroxiredoxin 1 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-Peroxiredoxin 1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Peroxiredoxin 1
Immunogen
Recombinant Human Peroxiredoxin 1 / PRDX1 protein (Catalog#11194-H08E)
Preparation
Produced in rabbits immunized with purified, recombinant Human Peroxiredoxin 1 / PRDX1 (rh Peroxiredoxin 1 / PRDX1; Catalog#11194-H08E; NP_002565.1; Met 1-Lys 199). Peroxiredoxin 1 / PRDX1 specific IgG was purified by Human Peroxiredoxin 1 / PRDX1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Peroxiredoxin 1 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Peroxiredoxin 1 Antibody (Rabbit Polyclonal antibody) Images

PRDX1 was immunoprecipitated using:

Lane A:0.5 mg A431 Whole Cell Lysate

2 µL anti-PRDX1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-PRDX1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 22 kDa

Observed band size: 22 kDa

Anti-PRDX1 rabbit polyclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:22 kDa

Observed band size:22 kDa

Anti-Peroxiredoxin 1 Antibody: Alternative Names

Anti-MSP23 Antibody; Anti-NKEF-A Antibody; Anti-NKEFA Antibody; Anti-PAG Antibody; Anti-PAGA Antibody; Anti-PAGB Antibody; Anti-PRX1 Antibody; Anti-PRXI Antibody; Anti-TDPX2 Antibody

Peroxiredoxin 1 Background Information

Peroxiredoxin-1, also known as Thioredoxin peroxidase 2, Natural killer cell-enhancing factor A, PRDX1, and PAGA, is a member of the ahpC/TSA family. Peroxiredoxin-1 is constitutively expressed in most human cells. It is induced to higher levels upon serum stimulation in untransformed and transformed cells. Peroxiredoxins (PRDXs) are a family of antioxidant enzymes that are also known as scavengers of peroxide in mammalian cells. The overexpression of Peroxiredoxin-1, which is one of the peroxiredoxins that is a ubiquitously expressed protein, was related to a poor prognosis in several types of human cancers. Peroxiredoxin-1 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin and may play an important role in eliminating peroxides generated during metabolism. Peroxiredoxin-1 Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The reduced Peroxiredoxin-1 expression is an important factor in esophageal squamous cancer progression and could serve as a useful prognostic marker.
Full Name
peroxiredoxin 1
References
  • Neumann, CA. et al., 2003, Nature 424 (6948): 561-5
  • Gisin, J. et al., 2005, J Clin Pathol. 58 (11): 1229-31.
  • Hoshino, I. et al., 2007, Oncol Rep. 18 (4): 867-71.
  • Cao, J. et al., 2009, EMBO J. 28 (10): 1505-17.

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