pCMV3-C-Myc

pCMV3-C-Myc Physical Map

pCMV3-C-Myc Physical Map Comments for pCMV3-C-Myc:
• CMV promoter: bases 250-837
• Enhancer: bases 838-1445
• SV40 early promoter: bases 2405-2774
• Hygromycin ORF: bases 2792-3817
• pUC origin: bases 4460-5133
• Kanamycin ORF: bases 5207-6022

Cloning Sites for ORF Inserting

Cloning Sites for ORF Inserting

Expression Vector pCMV3-C-Myc Information Description

Vector Name pCMV3-C-Myc
Vector Size 6164bp
Vector Type Mammalian Expression Vector
Expression Method Constitutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag Myc
Sequencing Primer Forward: T7(TAATACGACTCACTATAGGG); Reverse: BGH(TAGAAGGCACAGTCGAGG)

Myc Tag Information

A Myc tag is a polypeptide protein tag derived from the c-Myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A Myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the Myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.