- Positive control for the pCMV / hygro-Myc.
- Designed for mammalian expression, stable or transient.
- Hygromycin resistance gene for selection of stable cell lines.
pCMV / hygro-Positive Control Vector (C-terminal Fc-Myc tag) Physical Map
| Vector Name
||pCMV / hygro-Positive Control Vector (C-terminal Fc-Myc tag)
| Vector Size
| Vector Type
||Mammalian Expression Vector
| Expression Method
||Constitutive, Stable / Transient
| Antibiotic Resistance
| Selection In Mammalian Cells
| Protein Tag
| Sequencing Primer
pCMV / hygro-Positive Control Vector (C-terminal Fc-Myc tag) Sequence and Quality Control
1 ATGGGCTGGT CCTGCATCAT CCTGTTCCTC GTGGCGACCG CGACCGGGGT CCACAGC1GAG
61 CCCAAATCTT CTGACAAAAC TCACACATGC CCACCGTGCC CAGCACCTGA ACTCCTGGGG
121 GGACCGTCAG TCTTCCTCTT CCCCCCAAAA CCCAAGGACA CCCTCATGAT CTCCCGGACC
181 CCTGAGGTCA CGTGCGTGGT GGTGGACGTG AGCCACGAAG ACCCCGAGGT CAAGTTCAAC
241 TGGTACGTGG ACGGCGTGGA GGTGCATAAT GCCAAGACAA AGCCGCGGGA GGAGCAGTAC
301 AACAGCACGT ACCGTGTGGT CAGCGTCCTC ACCGTCCTGC ACCAGGACTG GCTGAATGGC
361 AAGGAGTACA AGTGCAAGGT CTCCAACAAA GCCCTCCCAG CCCCCATCGA GAAAACCATC
421 TCCAAAGCCA AAGGGCAGCC CCGAGAACCA CAGGTGTACA CCCTGCCCCC ATCCCGGGAT
481 GAGCTGACCA AGAACCAGGT CAGCCTGACC TGCCTGGTCA AAGGCTTCTA TCCCAGCGAC
541 ATCGCCGTGG AGTGGGAGAG CAATGGGCAG CCGGAGAACA ACTACAAGAC CACGCCTCCC
601 GTGCTGGACT CCGACGGCTC CTTCTTCCTC TACAGCAAGC TCACCGTGGA CAAGAGCAGG
661 TGGCAGCAGG GGAACGTCTT CTCATGCTCC GTGATGCATG AGGCTCTGCA CAACCACTAC
721 ACGCAGAAGA GCCTCTCCCT GTCTCCGGGT AAAGCT2GAGC AGAAACTCAT CTCAGAAGAG
1. Signal peptide
2. GCT was the nucleotide residue from the restriction site during plasmid construction, which has no influence on protein expression.
3. Myc Tag
Detect Positive Control Vector Expression by Western Blot
The 6 µg of plasmid was transfected into 20 ml of HEK293H suspension cells with Sinofection reagent (Cat# STF01). Experssion cells were cultured for 4d at 37°C (5% CO2). The 2×107 of cells were lysed in 1 ml of ice-cold modified RIPA Lysis Buffer with protease inhibitors cocktail (Sigma) by homogenization. The protein concentration of cell lysate was measured by BCA kit, and 1~5 µg of lysate were detected by western blotting using specific anti-tag antibody.