pCMV / hygro-Negative Control Vector (His-tagged)

  • Negative control for the pCMV / hygro-His clone.
  • sequence is the same as pCMV / hygro-His, except multiple cloning sites are reduced.
  • Designed for mammalian expression, stable or transient.
  • Hygromycin resistance gene for selection of stable cell lines.
 
pCMV / hygro-Negative Control Vector (His-tagged) Physical Map
 
Vector Sequence
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Description
 
 Vector Name pCMV / hygro-His
 Vector Size

5558bp

 Vector Type Mammalian Expression Vector
 Expression Method Constitutive, Stable / Transient
 Promoter CMV
 Antibiotic Resistance Ampicillin
 Selection In Mammalian Cells Hygromycin
 Protein Tag CACCATCACCACCATCATCACCACCATCAC
 Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

 

 
Schematic of pCMV / hygro-Negative Control Vector (His-tagged) Multiple Cloning Sites

  • Generation of a monoclonal antibody recognizing the heavily glycosylated CD45 protein and its application on identifying circulating tumor cells
    Author
    Zhang, W;Li, Z;Wang, Z;Yue, C;Zheng, H;Li, R;Zhou, M;Hu, Z;Wei, Z;Li, Q;
    Year
    2018
    Journal
    PLoS ONE
    Application
    cloning
  • Triptolide interferes with XRCC1/PARP1-mediated DNA repair and confers sensitization of triple-negative breast cancer cells to cisplatin
    Author
    Zhang, Z;Sun, C;Zhang, L;Chi, X;Ji, J;Gao, X;Wang, Y;Zhao, Z;Liu, L;Cao, X;Yang, Y;Mao, W;
    Year
    2019
    Journal
    Biomedicine & Pharmacotherapy
    Application
    expression
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